A joy that's shared is a joy made double


Protocol from 2016-08-30

Collaboration Freiburg

Participants Freiburg: Tina, Vivi, Chris, Kevin, Wlad
Paricipants Bielefeld: Cassandra, Judith, Fabian, Pascal, Mika
A first introduction to their projects was given from each team.
This year team Freiburg is working on a way for targeted drug delivery through bacterial spores. You can learn more about Team Freiburgs project here.

  • Exchanging Nanobodies with different binding properties
    • mCherry-/GFP binders were offered
  • Showed interest in our selection system
  • Offered measuring the binding affinity with the OCTET RED, a device to measure affinity of proteins
    • Minimal specifications for measurements were discussed (cloning into an expression vector and adding his-tags, as well as the purification of protein, etc.)

  • Exchanging Nanobodies with different binding properties
    • A part of our library was offered
  • We then talked about having more controls for the correction protein folding of their Nanobodies
    • Our Nabobody BIFC-Control was mentioned to them and suggested as well-suited for this part

We then agreed to exchange abstracts and discussed ways we could aid each other.

  • Freiburg: Think about having a control similar to our Nanobody BIFC-System.
  • Bielefeld: Think about getting the affinity of our binding proteins to specific targets measured by Team Freiburg.

Protocol from 2016-09-15

Participants Freiburg: Wlad
Paricipants Bielefeld: Judith, Mika
The projects were once again resumed and innovations were stated. We then came back to the topics we could help each other out.
  • The principle of the OCTET RED measurement and the conditions the proteins have to fulfill were mentioned once again.
    • It was stated that the antigen gets coupled with a biosensor for the measurement
    • He suggested that we should think of an additional exporter for secretion in the periplasm
    • Once again we talked about the protein purification and the necessity of a his-tag
    • The amount of time necessary for the correct condition was estimated.

  • Our Nanobody BIFC-Control was once again mentioned and the advantages were stated
    • We suggested sending them the two plasmids necessary for the BIFC containing the sequences for it.
    • We then informed them about the way they would be used and talked about how the split-proteins were designed and agreed on sending them the '.genious-file' and further details.
  • The conversation then switched back to the OCTET RED measurement Team Freiburg offered to carry out for us
    • We stated that the way of protein purification we chose (IMPACT) made tags unnecessary
    • We proceeded by telling them we would be able to use another way for defining the affinity between our target protein and our binder

  • We agreed to send them our Nanobody BIFC-control


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