Team:CSU Fort Collins/NoteBook/July
CSU iGEM
July
7/2/16:
- Ran PCR products from inter lab study colony PCR on a 1% gel
- No products
7/6/16:
- PCRed out Lrta from Synechocystis PCC 6003
- Re ran gel from inter lab study colony PCR on 1% gel
- No products
7/7/16:
- Ran Lrta PCR products on a 1% gel
7/8/16:
- Performed ligase cycling reaction on slr0168 and pSB1C3
- Ran all PCR products on 1%gel
- Digested Lrta(SpeI EcorI)
- Ligated Lrta and EYFP-LVA
- Transformed Lrta+EYFP-LVA ligation mixture
7/12/16:
- Dpn1 Digested slr0168 and pSB1C3
- Phosphorylated slr0168 and pSB1C3
- Performed ligase cycling reaction on phosphorylated mixture
- Transformed ligase cycling reaction products
- Digested EYFP-LVA(PstI)
- Ligated EYFP-LVA + LrtA
- Transformed EYFP-LVA+LrtA ligation mixture
- Performed colony PCR on 13 colonies with the Q1 block
- Ran PCR results on a 1.5% gel
7/13/16:
- Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies
- PCR amplified Q1 and Q2 for infusion cloning
7/14/16:
- Ran Q1 and Q2 blocks in a 1.5% gel
- Gel had no bands
- PCR amplified for infusion cloning
7/15/16:
- Ran Q1 and Q2 blocks in a 1.5% gel
- Infusion cloning on Quorum and PSB1C3
- Transformed infusion mixture
7/16/16:
- PCRed PSB1C3 for infusion cloning
- Ran PCR products on a 1.5% gel
- Infusion performed on Quorum gBlocks and PSB1C3
- Transformed infusion product
7/19/16:
- 2-step PCRed Qblocks together
- Ran PCR products on a 1.5% gel
7/20/16:
- Redid Qblock 2-step PCR
- Ran PCR products on a 1.5% gel
7/27/16:
- Q1,Q2,PSB1C3 were all PCRed for ligase cycling
7/27/16:
- Ran PCR products on a 1.5% gel
7/28/16:
- Digested Luc(ECORI XbaI) and Slr0118(ECORI XbaI)
- Ligated Slr0118 and Luc
- Transformed ligated mixture
7/29/16:
- Dpn1 DIGESTED Q1 and Q2 PCR products
7/30/16:
- Phosphorylated PSB1C3,Q1,and Q2 for ligase cycling
- Ligase cycling performed on phosphorylated mixture
- Transformed ligase cycling mixture
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