Team:CSU Fort Collins/NoteBook/June

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CSU iGEM



June

6/1/16:
  • Ran products from the Qblock colony PCR on a 1.5% gel
  • Transformed Bba_I712019 (Luc from firefly) into e. Coli
6/4/16:
  • Digested EYFP LVA(ecoRI xbai), and SLR0435(ecoRI xbai)
  • Heat inactivated at 80C
6/6/16:
  • PCRed PSB1C3 to prepare for infusion cloning
  • Ligated EYFP-LVA and SLR0435 overnight at 16C
6/7/16:
  • Dpn1 digested pSB1C3
  • Ran on 1.5% gel
  • Infusion on Quorum and PSB1C3
  • Transformed the infusion mixture
  • Transformed EYFP-LVA + SLR0435
6/8/16:
  • Performed colony PCR on 8 Qblock+PSB1C3 transformed colonies with phusion
  • Ran on 1.5% gel
  • Colony PCR on 16 colonies EYFP-LVA + SLR0435 with Taq
6/15/16:
  • Digested Slr0435(ECORI SpeI) and EYFP-LVA(xbai ECORI)
6/16/16:
  • Performed colony PCR on 15 additional colonies from Qblock+PSB1C3 transformed colonies
  • Performed colony PCR on BBa_1712019
6/17/16:
  • Ran Qblock+PSB1C3 PCR products in a 1.5% gel
6/20/16:
  • Ligated EYFP-LVA and SLR0435
  • Transformed EYFP-LVA+Slr00435 ligation mixture
  • Transformed DNA for interlab study
6/21/16:
  • Colony PCRed colonies for the inter lab study
6/22/16:
  • PCR amplified gBlock
  • PCR amplified PSB1C3 for infusion
  • Ran Colony PCR products from the 21 on a 1% gel
  • No Product
6/23/16:
  • Digested EYFP-LVA(xbai ECORI)
  • Ligated EYFP-LVA and Slr0435
  • Transformed EYFP-LVA+Slr0435
6/24/16:
  • Ran PCR products from the 22 on a 1.5% gel
6/25/16:
  • Repeated 2-step PCR a lower annealing temp
  • Colony PCRed interlab study colonies
6/26/16:
  • Ran Colony PCR products from the 25 on a 1% gel
  • No products seen on the gel
6/27/16:
  • Ran 2-step PCR products on 1.5% gel
  • No products seen on the gel
6/28/16:
  • Redid 2-step PCR with annealing temperatures 56C, and 53C
  • Colony PCR performed on 7 EYFP-LVA +SLR0435 transformed colonies
  • ReTransformed DNA for interlab study
6/29/16:
  • Ran result of EYFP-LVA+Slr0435 Colony PCR on 1% gel
6/30/16:
  • Used Strataclone Blunt pCR Cloning Kit to insert quorum gBlock into a vector in order for it to be sequenced
  • Performed colony PCR on the inter lab transformed colonies from the 28

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