Team:Danci-K8/Part Collection

CRISPR collection

Guide RNA for Cas9 for grapefruit juice bitterness flavor

Part:BBa_K2184001

Guide RNA for Cas9 for coffee biterness flavor

Part:BBa_K2184002

Guide RNA for Cas9 for NaCl & Sour flavor

Part:BBa_K2184003

Guide RNA for cas9 for umami & monosodium glutamate flavor

Part:BBa_K2184004

SNP for non-taster grapefruit juice bitterness taste with guide RNA for Cas9

Part:BBa_K2184032

SNP for non-taster coffee bitterness taste with guide RNA for Cas9

Part:BBa_K2184036

SNP for non-taster NaCl & Sour taste with guide RNA for Cas9

Part:BBa_K2184037

SNP for non-taster umami & monosodium gluten taste with guide RNA for Cas9

Part:BBa_K2184038

generate a synthetic DNA harboring the wildtype and mutant alleles (SNP) for the ability to sense taste which will be used as control for the Taqman assays and for preforming CRISPR on the mutant SNP allele, to convert it to the wildtype allele, as a feasibility test to be able to change people's taste sense and help them to consume necessary oral medications even if they taste very bitter to them.

Taqman assay on the Fluidigm nano-fluidic gene dynamic array chips (96:96) collection

SNP for PTC taster-TAS2R38 rs713598

Part:BBa_K2184005

SNP for sweet  taster-TAS1R2 rs35874116

Part:BBa_K2184006

SNP for grapefruit juice bitterness taster-TAS2R19 rs10772420‎‏

Part:BBa_K2184007

SNP for for NaCl & Sour  taster-TAS1R1 rs17492553

Part:BBa_K2184008

SNP consuming alcoholic taster-TAS2R16 rs846672

Part:BBa_K2184009

SNP PTC taster-TAS2R38 rs1726866‎‏

Part:BBa_K2184010

SNP for PTC taster-TAS2R38 rs10246939‎

Part:BBa_K2184011

SNP for coffee bitterness taster- TAS2R3 RS765007‎

Part:BBa_K2184012

SNP umami & monosodium gluten taster-TAS1R3 rs76755863

Part:BBa_K2184013

SNP coffee bitterness taster-TAS2R5 RS2234012‎‏

Part:BBa_K2184014

SNP for coffee bitterness taster-TAS2R5 RS2227264‎‏

Part:BBa_K2184015

SNP for cilantro preference taster-OR10A2 rs72921001‎‏

Part:BBa_K2184016

SNP for NaCl & Sour taster-TAS1R1 rs34160967‎

Part:BBa_K2184017

SNP for umami & monosodium gluten taster-TAS1R3 rs307377

Part:BBa_K2184018

SNP for coffee bitterness taster-TAS2R4 RS2234001

Part:BBa_K2184019

The ability of humans to sense food taste is driven in part by genetic polymorphisms in the taste receptor cells (TRCs) organized in taste buds located within gustatory papillae. Single nucleotide polymorphism (SNP) in genes encoding to taste receptors (belonging to the G-proteins coupled receptors family) have been found to influence peoples abilities to taste bitter (PTC, Coffee, Grapefruit juice), sweet, umami (Monosodium gluten), salts, Cilantro preference and more. People's ability to consume oral medications is driven in part by their sensitivity to drug bitterness.

We generate a SNPs gene panel- with those bio bricks which can determine people's preference of food tastes using Taqman assay on the Fluidigm nano-fluidic gene dynamic array chips (96:96). Endpoint fluorescent image data is acquired on the BioMark™ System for genetic analysis and data is analyzed using the Fluidigm SNP Genotyping Analysis software, to obtain genotype calls. In addition to saliva collection from students volunteering to participate, after completing their phenotypes forms of response to food tastes, we generate a synthetic DNA harboring the wildtype and mutant alleles (SNP) for the ability to sense taste which will be used as control for the Taqman assays.

Control for the Taqman assays and for preforming CRISPR on the mutant (non-taster) SNP allele collection

SNP PTC taster-TAS2R38 rs713598

Part:BBa_K2184020

SNP for sweet taster-TAS1R2 rs35874116

Part:BBa_K2184021

mutant SNP for non-taster consuming alcoholic flavor-TAS2R16 rs846672

Part:BBa_K2184022

mutant SNP for non-taster PTC flavor-TAS2R38 rs1726866‎‏

Part:BBa_K2184023

mutant SNP for non-taster PTC flavor-TAS2R38 rs10246939

Part:BBa_K2184024

mutant SNP for non-taster coffee bitterness flavor-TAS2R4 RS2234001

Part:BBa_K2184025

mutant SNP for non-taster coffee bitterness flavor-TAS2R5 RS2234012‎‏

Part:BBa_K2184026

mutant SNP for non-taster coffee bitterness flavor-TAS2R5 RS2227264‎‏

Part:BBa_K2184027

mutant SNP for non-taster coffee bitterness flavor-OR10A2 rs72921001‎‏

Part:BBa_K2184028

mutant SNP for non-taster NaCl & Sour flavor-TAS1R1 rs17492553

Part:BBa_K2184029

mutant SNP for non-taster umami & monosodium gluten flavor-TAS1R3 rs76755863

Part:BBa_K2184030

mutant SNP for non-taster grapefruit juice bitterness flavor-TAS2R19 rs10772420‎‏

Part:BBa_K2184031

mutant SNP for non-taster coffee bitterness flavor- TAS2R3 RS765007‎

Part:BBa_K2184033

mutant SNP for non-taster NaCl & Sour flavor-TAS1R1 rs34160967‎

Part:BBa_K2184034

mutant SNP for non-taster umami & monosodium gluten flavor-TAS1R3 rs307377

Part:BBa_K2184035

We generate a synthetic DNA harboring the wildtype and mutant alleles (SNP) for the ability to sense taste which will be used as control for the Taqman assays and for preforming CRISPR on the mutant SNP allele.

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