Team:ETH Zurich/Composite Part

COMPOSITE PARTS

Part Name Biobrick Comments
AHL regulated sfgfp expression BBa_K2116070 BBa_J23118 promoter with a downstream esabox, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHLregulated sfgfp expression BBa_K2116052 BBa_J23118 promoter with two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116071 BBa_J23118 promoter with three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116072 BBa_J23118 promoter with two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116073 BBa_J23118 promoter with three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116074 Nitric oxide responsive PnorV promoter with its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116075 Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116076 Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116077 Nitric oxide responsive PnorV promoter without its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116078 Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116079 Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116080 Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116081 Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116082 Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116083 Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
bxb1 integrase under Ptet promoter BBa_K2116056 bxb1 integrase with a very fast ssrA degradation tag, under the control of Ptet.
tp901 integrase under Ptet promoter BBa_K2116057 tp901 integrase with a very fast ssrA degradation tag under the control of Ptet.
superfolder gfp under Ptet promoter BBa_K2116085 sfgfp under the control of Ptet.
mNectarine under Ptet promoter BBa_K2116086 mNectarine under the control of Ptet.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116041 Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses sfgfp in presence of both nitric oxide and the AHL 3OC6HSL.
mNectarine under Ptet promoter BBa_K2116065 mNectarine with a moderately fast ssrA degradation tag, under the control of Ptet.
Dual reporter for integrase tp901 BBa_K2116058 Constitutive promoter J23118 flanked by attP and attB sites of tp901 integrase, initially expressing mNectarine. Once flipped, the reporter expresses sfgfp.
Dual reporter for integrase bxb1 BBa_K2116024 Constitutive promoter J23118 flanked by attP and attB sites of bxb1 integrase, initially expressing mNectarine with moderately fast ssrA degradation tag. Once flipped, the reporter expresses sfgfp.
bxb1 integrase under Ptet promoter BBa_K2116061 bxb1 integrase with a moderately fast ssrA degradation tag, under the control of Ptet.
tp901 integrase under Ptet promoter BBa_K2116062 tp901 integrase with a moderately fast degradation tag under the control of Ptet.
bxb1 integrase under Ptet promoter BBa_K2116063 bxb1 integrase under the control of Ptet.
tp901 integrase under Ptet promoter BBa_K2116064 tp901 integrase under the control of Ptet.
Nitric Oxide and AHL regulated bxb1 expression BBa_K2116059 Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses bxb1 with a moderately fast degradation tag, in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated bxb1 expression BBa_K2116028 Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses bxb1 in presence of both nitric oxide and the AHL 3OC6HSL.
Constitutively expressed transcriptional repressor EsaR BBa_K2116087 EsaR expressed under the control of constitutive promoter J23118. EsaR is responsive to the AHL 3OC6HSL.
Nitric oxide regulated sfgfp BBa_K2116088 Nitric Oxide responsive promoter controlling sfgfp expression.

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