Primer List
| No. | Primer name | Sequence 5'-3' | Tm[°C] |
|---|---|---|---|
| 1 | F-key sequence | CGGAATTCGCGGCCGCTTCTAGAGG | 67.9 |
| 2 | R-key sequence | GCCTGCAGCGGCCGCTACTAGTACC | 68.0 |
| 3 | F-message sequence | GCGTCGACGACCAAGCCTGCAAAAAC | 65.4 |
| 4 | R-message sequence | GCAAGCTTGTCGGTGGGTGCAATGC | 65.2 |
| 5 | F-qnrs1 e.coli | GCAAGCTTGAATTCGCGGCCGCTTC | 67.6 |
| 6 | R-qnrs1 e.coli | GCGGTACCCTGCAGCGGCCGCTACTAG | 70.9 |
| 7 | F-qnrs1 optimized | GCAAGCTTGAATTCGCGGCCGCTTC | 67.6 |
| 8 | R-qnrs1 optimized | GCGGTACCCTGCAGCGGCCGCTACTAG | 70.9 |
| 9 | amyE-prefix | GCGGAATTCGCGGCCGCTTCTAGATGTTTGCAAAACGATTCAAAAC | 73.6 |
| 10 | amyE-suffix | CGTACTAGTAGCGGCCGCTGCAGTCAATGGGGAAGAGAACCGCTTAAGC | 77.1 |
| 11 | F-gfp insert | GCAGATCTAAATTGAATTCAACGCTCGAATGC | 65.0 |
| 12 | R-gfp insert | GCGCTAGCATTACGCCAAGCTTGAATCTTGCTTG | 71.2 |
| 13 | F message sequencing | GCGCGTACGATCTTTCAGCCGACTC | 65.9 |
| 14 | R message sequencing | GCGCCGCGTTTCGGTGATGAAGAT | 65.3 |
| 15 | F key only amplify | GCGGACCAGCTCATGATTCTCAC | 60.9 |
| 16 | R key only amplify | GCGAGGAGGCTTACTTGTCTGCTTTCTTC | 65.9 |
| 17 | amyE int check | CTCTGCCAAGTTGTTTTGATAGAGTG | 59.2 |
| 18 | key only + prefix | GAATTCGCGGCCGCTTCTAGAGGACCAAGCCTGCAAAAC | 73.6 |
| 19 | key only + suffix | CTGCAGCGGCCGCTACTAGTAGTCGGTGGGTGCTATGGAGCGACAGAA | 78.3 |
| 20 | message prefix | GAATTCGCGGCCGCTTCTAGAGGACCAAGCCTGCAAAAACAAAG | 74.4 |
| 21 | message suffix | CTGCAGCGGCCGCTACTAGTAGTCGGTGGGTGCAATGCTTC | 76.0 |
| 22 | pATPI+prefix | GCGAATTCGCGGCCGCTTCTAGAGTATAGGTGAAAATGTGAACATTC | 72.9 |
| 23 | pATPI+suffix | CTGCAGCGGCCGCTACTAGTACAATTATCTGTCTCCTGATGAA | 73.0 |
| 24 | prefix sfGFP(Sp) | GAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAACTTTTTAC | 71.3 |
| 25 | suffix sfGFP(Sp) | CTGCAGCGGCCGCTACTAGTACATTATTATTTATAAAGTTCGTCCATAC | 71.0 |
| 26 | VF2 | TGCCACCTGACGTCTAAGAA | 55.4 |
| 27 | VR | ATTACCGCCTTTGAGTGAGC | 55.4 |
| 28 | nucA+prefix | GAATTCGCGGCCGCTTCTAGATGAAAAAGATTTGGCTGGCGCTGGCTG | 77.4 |
| 29 | nucA+suffix | CTGCAGCGGCCGCTACTAGTATTATTGACCTGAATCAGCGTTGTC | 74.2 |
| 30 | Gb_insert_F2 | GTACATCTTTGTAACTTTATTATACGACTGGGCCTTTCGTTTTATCTGTTG | 72.0 |
*prefix and suffix are underlined
