Team:HSiTAIWAN/Description
Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.
I know all the secrets of Chinese herbs and their magical healing powers.
If you are up to a challenge,find me at team HSiTW at the jamboree.
I am the one in a straw hat,showing them pearls.I will be waiting.
Muhahahahaha
Hi there! My name is Nu Zhen Chi. This is how I look like.
Take a closer look; guess which part of me is used as medicine?
(1) the root 
(2) the stem
(3) the leaf
(4) the seed
Ans.(4) the seed
Name: 女貞子 (Nu Zhen Chi)
Botanical Name: Ligustrum lucidum Aiton
I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,
lower back pain, premature graying of the hair and tinnitus.
Hello! My name is Chuan Xiong. This is how I look like.
Make a guess, which part of me is used as medicine?
(1) the root 
(2) the stem
(3) the leaf
(4) the seed
Ans.(1) the root
Name: 川芎 (Chuan Xiong)
Botanical Name: Ligusticum chuanxiong Hort
I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.
In addition, I relieve you of physical pain,
such as headaches, abdominal aches, chest pain, and muscle pain.
Finally, I free the ladies of menstrual disorders and amenorrhea.
What’s up? My name is Dang Gui. I can:
(1) stop coughing 
(2) regulate mense
(3) reduce internal heat
Ans.(2) Regulate mense
Name: 當歸 (Dang Gui)
Botanical Name: Angelica sinensis (Oliv.) Diels
I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.
Reference
臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北
Non-Profit Organization Brion Research Institute of Taiwan.
Chinese Herb Gallery. Jade Institute
Herbal Glossary. Shen-Nong- Chinese Traditional Medicine
Acknowledgement
Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.
Description
- Improving Existing Parts
- Autoregulation circuit
Description
Description of Improving Previously Existing Parts/Devices
In our project, we have done improvement of the function/characterization on two previously existing BioBrick Part/Device(BBa_I721002 and BBa_K1613017)
(1).Design of Circuit
It is a lead binding protein (PbrR), belonging to a MerR family. It plays a role of regulator in gene transcription of lead resistance operon. In order to measure the generation of PbrR, we designed two types of PbrR genetic circuits to measure the PbR according to the GFP expression downstream.
(a)Autoregulated PbrR circuit(BBa_K1961004): an autoregulated generator of PbrR measured by GFP expression downstream. PbrR and GFP expression is under the control of TetR and promoter PTet (Figure 1(a))
(b)Constitutive PbrR circuit(BBa_K1961005): a constitutive generator of PbrR measured by GFP expression downstream. PbrR and GFP expression is under the constitutive promoter (Pcon, BBa_J23102 ) (Figure 1(b))
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| Figure 1(a). The autoregulated PbrR circuit | Figure 1(b). The constitutive PbrR circuit |
In order to investigate the response of PbrR in these two circuits, we used GFP as a reporter downstream as a proxy to measure the PbrR produced by two circuits. For autoregulation PbrR circuit, we want to study in detail about the response of PbrR protein with increasing amount of inducer tetracylcine. The advantage of this autoregulation circuit is to get very precisely gradual response to the inducer, which implies that the amount PbrR protein will be a smooth function of inducer. In contrast, we also designed the constitutive PbrR generator. This circuit enables us to measure the background expression of PbrR in E. Coli. This will help us to compare and recognize the generation of PbrR under autoregulation. All the constructs used here, the Hill coefficient will be calculated to disclose the intrinsic property of genetic circuits and be a guide for our biosensor project.
(2).Simulation Result of Autoregulated PbrR generation
Figure 2. The Autoregulation circuit and the related rate equations
Autoregulation circuit
Figure 3. The parameter space for constitutive circuit
Figure 4. The fold analysis for autoregulation and constitutive circuits
This was a previously existing composite part can detect lead ions. In 2015, HSNU-TAIPEI didn’t succeed and didn’t ship this part. This year, we re-do the ligation of this composite part as new part (BBa_K1961006) and tested its fluorescence.
Figure 7 Fluorescence of Lead Biosensor in different concentrations of lead solution (Part BBa_K1961006)
Figure 8 Fluorescence/OD of Lead Biosensor in different concentrations of lead solution(Part BBa_K1961006)
