Team:HSiTAIWAN/Notebook/FA/CYP

X

　Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.
　I know all the secrets of Chinese herbs and their magical healing powers.
　If you are up to a challenge,find me at team HSiTW at the jamboree.
　I am the one in a straw hat,showing them pearls.I will be waiting.
　Muhahahahaha

　Hi there! My name is Nu Zhen Chi. This is how I look like.
　　Take a closer look; guess which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(4) the seed
　Name: 女貞子 (Nu Zhen Chi)
　Botanical Name: Ligustrum lucidum Aiton
　I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,
lower back pain, premature graying of the hair and tinnitus.

　Hello! My name is Chuan Xiong. This is how I look like.
　Make a guess, which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(1) the root
　Name: 川芎 (Chuan Xiong)
　Botanical Name: Ligusticum chuanxiong Hort
　I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.
　In addition, I relieve you of physical pain, such as headaches, abdominal aches, chest pain, and muscle pain.
　Finally, I free the ladies of menstrual disorders and amenorrhea.

　What’s up? My name is Dang Gui. I can:
(1) stop coughing
(2) regulate mense
(3) reduce internal heat

　Ans.(2) Regulate mense
　Name: 當歸 (Dang Gui)
　Botanical Name: Angelica sinensis (Oliv.) Diels
　I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.

　　Reference
　臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北
　Non-Profit Organization Brion Research Institute of Taiwan.
　Chinese Herb Gallery. Jade Institute
　Herbal Glossary. Shen-Nong- Chinese Traditional Medicine
　　Acknowledgement
　Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.

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  • Functional Array

    • Pb
    • Cu
    • As
    • CYP

  • Chinese Medicine testing

    • Pb
    • Cu
    • As
    • CYP

    • Functional Array CYP

    2016/05/19

    Topic:畫菌Streak Culture、prepare Chinese herb solutionMaterials

    Material:

    1.Streak Culture

    　菌bacteria、agardish、畫菌loop、alcohol Burner、Alcohol、napkin

    2.prepare Chinese herb solution

    　木香auckandia root、岩精、朱砂cinnabar

    Eppendorf*2

    15ml tube*1

    ddH2O

    Procedure:

    Streak Culture

    　1.在需要做實驗的範圍內噴灑酒精，再以紙擦乾，已完成一個簡易無菌室

    　2.將畫菌筆以酒精燈消毒

    　3.以畫菌筆挖取菌，塗抹在新agardish

    　4.重複步驟2、3直到畫完一個圓周為止

    　5.封膜後放入4°C冷藏室。

    Streak Culture

    取朱砂、木香各1.5mg、岩精取15mg

    將朱砂、木香溶入1.5ml的ddH2O、岩精溶入15ml的ddH2O

    封膜後放入4°C冷藏室。

    2016/07/28

    Objecive:

    To study the E. coli DH5αgrowth rates inSOC由於之後要做在中藥處理下，DH5α生長曲線的變化，為了先知道DH5α大概的生長情形，什麼時候會到達停滯期，以及一開始需不需要每半小時就測一次，所以先做這個pre-test，以為後面的實驗鋪路。

    Material:

    DH5α pcDNA (ctrl)on SOCagar plates

    SOC broth

    Amp (1000 X)

    15 ml tube、50 ml tube

    96 well plate

    Procedure:

    7/27

    　1.Culture

    　　下午5:00 開始養一管母菌，2 ml SOCbroth (with Amp)，37shaker(見附件氧菌步驟)

    　2.隔天早上測OD595


    7/27

    前置作業

    　1.08:30取1 ml菌液到新的15 ml 管子，加入37°C 1 ml SOCbroth (with Amp)，37°C shaker2小時

    　2.開始養菌，以50 ml 管子裝菌液，為了測試做三種濃度：以SOC稀釋菌液至10、20、50、100、200倍，，再加分別加入原始菌液，形成五管30ml的菌液。最後個加入30ul的AMP。

    　3.將五管裝有30ml菌液的50ml試管放入37°C shaker，每30分鐘測一次吸光值，每次測吸光值都要測blank(LB broth)。

    2016/08/19

    Topic:H2O2 Testingover night

    Material:

    E. coli(dh5a+SOS plasmid K079050)

    SOC medium

    CP (antibiotic)

    H2O2 solution(0.25mM、0.50mM、0.75mM、1mM、1.25m)

    懸浮液

    Pipette

    Eppendorf

    15ml centrifuge tube

    37°C shaker

    Procedure:

    Day1

    　1.Culture 6 tubes of E. coli(dh5a+SOS plansmid K079050), each tube contains SOC, bacteria, 2ul AMP, and different cocentrations of H2O2 solution (0.25mM、0.50mM、0.75mM、1mM、1.25m)

    　2.Incubate the bacteria overnight.


    Day2

    　1.Take out the six tubes

    　2.Load 1.5 ml of each tube into six different eppendorfs

    　3.Centrifuge the eppendorfs for 5min.

    　4.Draw out the supernate of the eppendorfs and leave the bacteria precipitate in the eppendorf

    　5.Use the 500 ul 懸浮液 to solute the bacteria precipitate

    　6.Load 200ul of the six kinds of bacteria liquid made in step5 into 不透明96well盤

    ．Examine the fluorscence.(Excitation max485 Emission max520)not certain

    Results:

    E. coli in three eppendorfs were killed. The negative control group shows flourescence. We think it is contaminated. Thus, we will repeat this pretest again

    2016/08/22

    Topic:H2O2 Medium Testing(20 minutes)

    Material:

    E. coli(dh5a+SOS plasmid K079050)

    SOC medium

    CP (antibiotic)

    H2O2 solution(0.25mM、0.50mM、0.75mM、1mM、1.25m)

    懸浮液

    Pipette

    Eppendorf

    15ml centrifuge tube

    37°C shaker

    Procedure:

    　1.Culture 6 tubes of E. coli(dh5a+SOS plansmid K079050), each tube contains SOC, bacteria, 2ul AMP, and different cocentrations of H2O2 solution (0.25mM、0.50mM、0.75mM、1mM、1.25m)

    　2.Incubate the bacteria 20 minutes

    Day 2

    　1.Take out the six tubes

    　2.Load 1.5 ml of each tube into six different eppendorfs

    　3.Centrifuge the eppendorfs for 5min.

    　4.Draw out the supernate of the eppendorfs and leave the bacteria precipitate in the eppendorf

    　5.Use the 500 ul 懸浮液 to solute the bacteria precipitate

    　6.Load 200ul of the six kinds of bacteria liquid made in step5 into 96wellplate

    ．Examine the fluorscence.(Excitation max485 Emission max520)not certain

    Results:

    2016/09/01

    Topic:H2O2 Medium Testing(50min-70min-90min)

    Material:

    E. coli(dh5a+SOS plasmid K079050)/p>

    SOC medium

    CP (antibiotic)

    H2O2 solution(0 mM, 1mM,1.25mM, 1.5mM, 1.75mM), blank

    懸浮液

    Pipette

    Eppendorf

    15ml centrifuge tube

    37°C shaker

    Procedure:

    Part1

    　1.Culture 6 tubes of E. coli(dh5a+SOS plansmid K079050), each tube contains SOC, bacteria, 2ul AMP, and different cocentrations of H2O2 solution (0.25mM、0.50mM、0.75mM、1mM、1.25m)

    　2.Incubate the bacteria 50 minutes, +20 minutes, +20 minutes

    Part2

    　3.Take out the six tubes

    　4.Load 1.5 ml of each tube into six different eppendorfs

    　5.Centrifuge the eppendorfs for 5min.

    　6.Draw out the supernate of the eppendorfs and leave the bacteria precipitate in the eppendorf

    　7.Use the 500 ul懸浮液to solute the bacteria precipitate

    　8.Load 200ul of the six kinds of bacteria liquid made in step5 into 96wellplate

    Results: