Team:IISc Bangalore/Safety

Team IISc iGem

Safety

Our Safety Training

Safety In Our Lab

Biological Labs are assigned to specific Instructors and Professors in our institute. Each lab has a lab manager who looks after the proceedings of the lab during the working hours. He/ She is responsible for the safety of biology labs at our institution.
The guidelines for laboratory depend on the type of lab. The lab that we work in is a Low-Risk Laboratory and all the general guidelines that apply to a low risk laboratory are valid for us. Some them include:
1) No consumption of Food inside the lab premises.
2) Decontamination of equipment and work spaces with spirit or heat as appropriate.
3) Use of laboratory coat.
4) Careful handling of sharp equipment.
5) Mechanical pipetting only.
6) Proper disposal of microorganisms after completion of experiment.
7) Washing hands after experiments.
8) Proper Training to all lab members for the event of a fire.

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Our Safety Trainingh1>
We learnt the following in our safety training: -
1) Orientation of lab: We are extensively told about the location of the cold room, freezers, incubators, LAFs, fire exit and various equipment we might need in the course of our work(Micropipettes, Beakers, Eppendorfs, falcons,test-tubes, etc.)
2) Use of Equipments: We were taught how to use the LAF (UV treatment for 15 minutes, Wiping of work surface with ethanol, keeping the area in front of the air flow empty, etc.), the Cold Room (How to control the temperature and accessing the safety latch in case we are stuck inside and the door locks), the Autoclave machine, the fire extinguisher, the centrifuge, the incubators .
3) General Lab Etiquette: We were given instructions of how to use various hand held equipment: The micro pipettes, the glass spreaders, the Bunsen burners. What practices to adopt when working inside of the LAF (keeping proper ventilation, sterilizing everything, washing hands with alcohol,etc.). We were also informed of some other general rules of a Level 1 Bio safety Lab (Not storing or consuming food inside of the lab, avoiding splashing, etc.).
4) Protocol Specific Instructions: As our team comprised of undergraduates from various fields of work, our instructor patiently explained the small subtleties of all common protocols that we would follow during the summer. He gave us training for Transformation(How to operate the Centrifuge and the Heater), preparation of competent cells, plate spreading, etc. and also told us where and how we should store the things that we will require over the course of our work.
5) Fire Event: We were given instructions as to what we should do in case of a fire in the lab. We were provided with the contact numbers of the campus security and were also taught how to use a fire extinguisher.

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