Team:IIT-Madras/Composite Part
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from BBa_K1956001, BBa_K1956002, BBa_K1956003, BBa_K1956004.
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from BBa_K1956014-BBa_K1956015, BBa_K1956017-BBa_K1956018, BBa_K1956022-BBa_K1956023.
3. RIBOS devices: We have cloned RIBOSON, BBa_K1956019 and RIBOSOFF, BBa_K1956005, BBa_K1956020, BBa_K1956021 devices to test their strength and functionality.
4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: BBa_K1956006, BBa_K1956007, BBa_K1956008, BBa_K1956009, BBa_K1956010, BBa_K1956011, BBa_K1956012, BBa_K1956013.
Best Composite Part
Dual expression vector, BBa_K1956016, device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
