Seventeen biobricks were produced, in two part collections, one a magnetosome collection and the other an amyloid collection. The majority of the parts were cloned into the pSB1C3 backbone using either Gibson assembly or restriction enzyme ligations. Some of parts were cloned into pSB1A3 because a co-transformation with a chloramphenicol resistant plasmid containing a cytochrome maturation factor is required for the parts to function, or because they needed to be transformed into VS45 E. coli strain that also contains a chloramphenicol resistant plasmid. The genes were initially cloned into plasmids containing an RFP indicator (BBa_JJ04450), which allowed for easy visualisation of correct colonies.

Name	Type	Description	Designer	Length
Bba_K1985000	Coding	pSB1C3-MamP	Jasmine Cornish	810 bp
Bba_K1985001	Coding	pSB1C3-MamT	Jasmine Cornish	525 bp
Bba_K1985002	Coding	pSB1C3-MamX	Jasmine Cornish	810 bp
Bba_K1985003	Coding	pSB1C3-[SecS-sol-MamP]	Jasmine Cornish	837 bp
Bba_K1985004	Coding	pSB1C3-[SecS-sol-MamT]	Jasmine Cornish	546 bp
Bba_K1985005	Coding	pSB1C3-[SecS-sol-MamX]	Jasmine Cornish	780 bp
Bba_K1985007	Coding	pSB1A3-[AraC pBAD]-MamO	Jasmine Cornish	3115
Bba_K1985017	Coding	pSB1A3-[AraC-pBAD]-MamOP	Jasmine Cornish	3931 bp
Bba_K1985008	Coding	pSB1A3-[AraC-pBAD]-MamOPX	Jasmine Cornish	4747 bp
Bba_K1985009	Coding	pSB1A3-[AraC-pBAD]-MamOPXT	Jasmine Cornish	5278 bp
Bba_K1985010	Coding	pSB1C3-[CsgA-SS-Sup35-1-61]	Rita Adriani	321 bp
Bba_K1985011	Coding	pSB1C3-[CsgA-SS-Sup35-1-61-Cytb562]	Rita Adriani	669 bp
Bba_K1985012	Coding	pSB1C3-[CsgA-SS-Sup35NM]	Hanya Benotmane	960 bp
Bba_K1985013	Composite	pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35-1-61]	Rita Adriani	1227 bp
Bba_K1985014	Composite	pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35-1-61-Cytb562]	Rita Adriani	1232 bp
Bba_K1985015	Composite	pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM]	Hanya Benotmane	1229 bp
Bba_K1985016	Regulatory	pSB1A3 AraC pBAD	Rita Adriani	1210 bp
Bba_K1985006	Coding	pSB1C3-MamO	Jasmine Cornish	810 bp

Team:Kent/Parts

Overview