# Team:Manchester/Model/GlucoseOxidaseReaction

Manchester iGEM 2016

# Glucose Oxidase Reaction

Oxidation of glucose to Glucono-δ-lactone and Hydrogen Peroxide using Glucose Oxidase (EC 1.1.3.4)

Chemical Equation

$${Glucose} + {Oxygen} \rightleftharpoons {Glucano-} \delta {-lactone} + {Hydrogen} \ {Peroxide}$$

Rate Equation

$$\nu = \frac{K_{cat}[GOx] \frac{[Glucose]}{K_{m,Glucose}}\left({ 1- \frac{[Glucose]}{[H_2 O_2][{GDL}]} \frac{1}{K_{eq}}} \right)}{1+\frac{[Glucose]}{K_{m,Glucose}}+\frac{[H_2 O_2]}{K_{m,H_2 O_2}}+\frac{[H_2 O_2][GDL]}{K_{m,GDL}K_{m,H_2 O_2}}+\frac{[GLD]}{K_{m,GDL}}}$$

where:

Symbol Meaning
$$K_{m,A}$$ Michaelis constant of species A
$$K_{cat}$$ Turnover number
$$[A]$$ Concentration of A
$$\nu$$ Reaction Rate

Parameter Sourcing

Kcat and Km parameters were collected from BRENDA

Keq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from MetaCyc

$$K_{eq} = e^{\frac{-\Delta G} {RT}}$$

Parameters with uncertainty

Km

Uncertainty in Km is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property Value
Minimum 0.815 mM
Maximum 733 mM
Mean 43.3 mM
Parameters Sourced 88

Kcat

Uncertainty in Kcat is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property Value
Minimum 0.03 s-1
Maximum 2003 s-1
Mean 617.3 s-1
Parameters Sourced 33

Keq

Uncertainty in Keq is introduced using a uniform range of temperature variations (298 K ± 5 K). Properties of this can be seen in the table below:

Property Value
$$- \Delta G$$ 140.833456736 kJ mol-1
Minimum Temperature 293 K
Maximum Temperature 303 K