Team:NRP-UEA-Norwich/Parts

NRP-UEA-NORWICH iGEM

Parts

All BioBricks consist of coding sequences for hydABC and hyaABC subunits for the FeFe & NiFe hydrogenase enzymes from Shewanella oneidensis MR-1. These were ligated into the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism Shewanella oneidensis MR-1 and were submitted to the iGEM registry on the 19th of October.

Name in Registry Backbone Gene expressed Function Length (bp)
BBa_K1957001 pSB1C3 hydC (Fdh) FeFe hydrogenase outer periplasmic subunit 705
BBa_K1957002 pSB1C3 hydB (SII C-terminus) FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus 374
BBa_K1957003 pSB1C3 hydB (SII N-terminus) FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus 374
BBa_K1957004 pSB1C3 hydA FeFe hydrogenase membrane bound catalytic subunit 1266
BBa_K1957005 pSB1C3 hyaA NiFe hydrogenase central subunit 1156
BBa_K1957006 pSB1C3 hyaC NiFe hydrogenase outer periplasmic subunit 695
BBa_K1957007 pSB1C3 hyaB (SII C-terminus) NiFe hydrogenase membrane bound catalytic subunit 1771
BBa_K1957008 pSB1C3 hyaB (SII N-terminus) NiFe hydrogenase membrane bound catalytic subunit 1771
BBa_K1957009 pSB1C3 hydABC, FeFe Hydrogenase gene cluster (SII N-terminus) FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus 2291

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