Team:NTHU Taiwan/Parts

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Parts

Basic part:

Promoter:

Name	Part name	function
Lac1	BBa_R0010	IPTG promoter

RBS:

Name	Part name	function
Elowitz 1999	BBa_B0034	RBS

Coding:

Name	Part name	function
Fac-dex1	BBa_K2073000	fluoroacetate dehalogenase
Fac-dex3	BBa_K2073001	fluoroacetate dehalogenase
Crcb-flc	BBa_K2073002	fluoride transporter
Crcb-bpe	BBa_K2073003	fluoride transporter
Crcb-La1	BBa_K2073004	fluoride transporter
Crcb-La2	BBa_K2073005	fluoride transporter
Mut3b	BBa_E0040	GFP

Terminator:

Name	Part name	function
Terminator	BBa_B0012	Terminator

Composite Parts:

Name	Part name	function
fac-dex1 with IPTG promoter	BBa_K2073008	Express fac-dex1
fac-dex3 with IPTG promoter	BBa_K2073009	Express fac-dex3
Crcb(flc) kit	BBa_K2073010	express Crcb(flc)
CRCB(bpe) kit	BBa_K2073011	express CRCB(bpe)
CrcB-La1 kit	BBa_K2073012	express CrcB-La1
CrcB-La2 kit	BBa_K2073013	express CrcB-La2

Team part:

Main part:

Part BBa_K2073008:

Part BBa_K2073009:

Both Fac-dex1 and Fac-dex3 are fluoroacetate dehalogenases. The enzyme can break down the carbonfluorine bond from PFOA/PFOS.

In this part we use IPTG promoter BBa_K2073009 to control the protein expression of Fac-dex.

Part BBa_K2073010, BBa_K2073011, BBa_K2073012, BBa_K2073013:

Fluoride has dramatic effects on most of bacteria. Bacteria would fend off fluoride, which is organisms treat as toxic substance, by throwing it out. Ion transporters is the common used method.

BBa_K2073010~ BBa_K2073013 can express different fluoride transporters(CrcB family) in E.coli. CrcB can be transported to plasma membrane and expulse fluoride ion from bacteria . Avoid bacteria die due to fluoride.

Test part:

Part BBa_J04430:

We use fluorescence microscope to measure GFP fluorescence. In this part we want to know how bacteria look like when it express GFP.

Part BBa_K2073007:

In this part we use intensity of GFP to measure the protein expression of Fac-dex3. If the engineered bacteria shows GFP fluorescence, we will know that the Fac-dex3 is successfully expressed by bacteria.