Team:Northwestern/06 24

Notebook

Friday, June 24th

Agenda:

  • Split Cas9 construct and research
  • Review experiment presentation
  • Research questions
  • Safety sheets
  • Interlab
  • Edit GenScript grant
  • Integrated human practices
  • Website
  • iGEM kit DNA concentration?
  • Go through kit, look for enzymes

Tasks:

Sara

  • Searched for rapamycin-inducible dimer Cas9
  • Helped with some of the Gibson Cas9 constructs
  • Watched Genome Compiler tutorials

Paul

  • Worked on presentation

Tasfia

  • Worked on GenScript
  • Helped Jordan with iGEM Interlab data colllection
  • Helped Sara make Cas9 split parts and modified Tat and Sec parts on Genome Compiler

Michelle

  • Searched the iGEM 2016 plates for practice material
  • Website CSS reading
  • Research on OMV formation and uptake for Patrick

Tyler

  • Revised presentation/experimental procedure
  • Redesigned proteins fusions
  • Ran Gibson on split Cas9, generated usable primers
  • Met with Promega, emailed them needed products

Shu

  • Sorted out the stuff in the freezer
  • Researched on potential membrane proteins

Sam

  • Reviewed the presentation and prepared to present
  • Presented to Jewett
  • Wrote to Jennifer
  • Got worried about the DNA concentrations and whether we need to PCR more DNA, and as a result, whether we need to design a bunch of primers ASAP
  • Helped edit GenScript

Jordan

  • Sampled the Interlab cell culture every hour for six hours
  • Interlab almost done!
  • Looked a little bit at the GenScript app
  • Watched some Genome Compiler tutorials

×

Loading ...