Team:Northwestern/06 27

Notebook

Monday, June 27th

Agenda:

  • Revise GenScript
  • Plate-read Interlab
  • Competent Cell efficiency test
  • Practice cloning
  • Take an inventory on the freezer
  • Make more LB

Tasks:

Sara

  • Emailed Dr. Zhang asking about rapamycin-inducible split Cas9
  • Got a reply! They’ll send us a plasmid and the gene map
  • Helped with constructs

Paul

  • Competent Cell test kit transformation
  • Research background information (other delivery methods) for the GenScript grant

Tasfia

  • GenScript
  • Comp cell efficiency test with Paul

Michelle

  • Revised GenScript
  • Some CSS research
  • Talked with Dr. Mordacq and Jordan about Interlab plate reading

Tyler

  • Ordered BB Primers
  • Began redesigning Cas9 split optimized for E. coli
  • Checked against other saCas9 sequences
  • High-level design of all constructs
  • Added sources to Gen Script Doc

Shu

  • Made an inventory list of stuff in the freezer
  • Did some research for Genscript and helped edit it

Sam

  • Edited GenScript
  • Tested 37°C LUDOX
  • Emailed Purdue
  • Checked freezer for primers
  • Emailed Mitch about primers
  • Wrote bio
  • Read some of the source papers for the review paper that sparked our whole project + added them to Mendeley

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