Team:Northwestern/06 30

Notebook

Thursday, June 30th

Agenda:

  • Constructs
  • Discuss things with Purdue
  • Protocol Practice- miniprep and digestion
  • Crowdfunding
  • Presentation practice

Results:

  • Nothing in the gel except the ladder was visible under UV light
  • The ImageJ program was not working
  • Left the gel out too long in the light and our SybrGreen degraded

Tasks:

Michelle

  • CSS studying
  • Tat & Sec sequence selection
  • Miniprepped practice plasmids

Sam

  • Wrote about things to discuss with Purdue

Sara

  • Did the digest and ran the gel
  • Emailed Bernd about split Cas9

Tasfia

  • ClyA construct
  • Miniprep practice

Tyler

  • Communicated w/sponsors (Promega and Invitrogen)
  • Worked on split Cas9 project, designed overlaps, need to check with Bradley

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