Team:Northwestern/07 01

Notebook

Friday, July 1st

Agenda:

  • Constructs
  • Practice presentations
  • Ligation
  • Meeting with grad students

Results:

Cloning practice: Redid the gel from yesterday. This time, we didn't run the gel quite long enough and the bands didn't separate that much.

Tasks:

Michelle

  • Website research
  • Golden Gate preparation

Sara

  • Ran the gel again
  • Emailed Bernd
  • Looked into choosing homologous regions to add onto the signaling sequences, ran into problems with annealing temp

Tasfia

  • Attempted to make overhangs on SS parts, but the Tm was too high for optimal Gibson assembly with really long overhangs
  • Cloning practice

Tyler

  • Finalized homology arms for split Cas9
  • Designed RTW PCR primers of tet backbone
  • Received Promega’s order of restriction

Jordan

  • Helped with gels
  • Crowdfunding got approved
  • Edited crowdfunding page
  • Presented to grad students

Paul

  • Update presentation with GFP fusions
  • Researched GFP fusions
  • Upload sfGFP to Genome Compiler

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