Team:Northwestern/07 05

Notebook

Tuesday, July 5th

Agenda:

  • Meet with Taylor to make legit comp cells (11 am)
  • Skype the Purdue iGEM team (1 pm)
  • Use the plate reader for the Interlab
  • Make Golden Gate primers
  • Work on Gibson primers and overhangs

Tasks:

Jordan

  • Made competent cells with Taylor
  • Crowdfunding stuff
  • Made Experiment centrifuge video

Michelle

  • Prepared competent cells aliquots with Taylor
  • Website reading
  • Finished ClyA homology
  • Created signaling sequence homology
  • Checked existing primers and constructs

Paul

  • Designed GFP Golden Gate Primers
  • Designed GFP flexible linker primers
  • Communicated w/ Purdue
  • Got familiar w/ Genome Compiler

Sam

  • Plate reader for Interlab
  • Researched potential outreach people
  • Researched Gibson assembly
  • Talked to Purdue
  • Mosquito forum stuff for Building with Biology outreach

Sara

  • Worked on Golden Gate constructs
  • Went to the Skype call with Purdue

Tasfia

  • ClyA plasmid and homology
  • Prepared competent cells that hopefully actually work this time
  • Updated slideshow
  • Researched Golden Gate assembly

Tyler

  • E-mailed Feinberg about free sequencing
  • Made slight changes to split Cas9 sequence
  • Generated Primers for opening plasmid at SS (have to redo tomorrow because change in RBS sequence)
  • Reviewed signal sequences with homology arms

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