Team:Northwestern/07 11

Notebook

Monday, July 11th

Agenda:

  • Do more research on/ask more grad students for a gRNA plasmid for reporter
  • Cloning practice
  • Email grad students
  • Linearize Tet plasmid
  • Genedrive prep
  • Group picture
  • Pick up the plates

Tasks:

Michelle

  • CSS research

Paul

  • Researched gene drives
  • PCR for linearizing Tet backbone

Sam

  • Researched gene drives
  • Learned about alternative Cas systems (other than Cas9)
  • Learned CSS

Sara

  • Learned about malaria
  • Prepared for the gene drive meeting tomorrow
  • Read more about Cas9

Tasfia

  • Linearized Tet backbone
  • Wrote lab notes for Experiment

Tyler

  • Ordered BB Primers
  • E-mailed CGM and received confirmation on running 100-200 sequencing reactions
  • Looked into possible experiments for testing functionality of Cas9 (pulled together material for a possible mRFP test)
  • Finalized sequencing primers for Cas9, sent them to Kelly
  • Watched a few videos of the basics of simbiology
  • Began to read about saCas9 and how it functions

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