Team:Northwestern/07 14

Notebook

Thursday, July 14th

Tasks:

Michelle

  • Worked on CSS on menu
  • Studied javascript

Paul

  • Investigated DNA gels
    • Made gels using our TAE & our agarose, Kelly’s TAE & agarose, our TAE & Kelly’ agarose, Kelly’s TAE & our agarose
    • Our agarose is the problem
    • 3 batches of agarose made from the 3 bottles in the lab
    • We’ve been using Low Melting Point agarose by mistake
  • Prepared miniprep culture

Sam

  • Learned even more CSS
  • Started learning JavaScript

Sara

  • Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night
    • Ran a miniprep for each tube of culture, one with water and one with elution buffer
    • 89 ng/uL with water elution, 25 ng/uL with elution buffer elution
  • Made 3 small test containers of 1% agarose
  • Made a Primers and Parts tab in the lab notebook

Shu

  • Investigated gel
  • Edited the assembled parts
  • Learned how to make animation of proposed pathways

Tasfia

  • Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night
    • Ran a miniprep for each tube of culture, one with water and one with elution buffer
    • 89 ng/uL with water elution, 25 ng/uL with elution buffer elution
  • Tested different containers of agar for use in 1% agarose gels
  • Published a note on Experiment
  • Wrote more content drafts for website
  • Made a list of graphics we need

Tyler

  • Finished mRFP and mRFP-gRNA constructs, sent them to grad students
  • Sponsor communication (Bio-rad and Qiagen)
  • Created meeting powerpoint for tomorrow

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