Team:Northwestern/08 07

Notebook

Sunday, August 7th

Tasks:

Sara & Tyler

  • Picked colonies on MCherry 1-5 plates from 8/4 and MCherry 1 from 8/5
  • Because Tet plates were contaminated, used a higher concentration of tetracyline than previous, 20 uL of 5 mg/ml, working concentration 50 ug/ml 2 ml cultures of each
  • MCherry and GFP storage vector plates had lawns on them, and lawns on the negative control--plates were irregularly poured or antibiotic is bad, could be both
  • Antibiotic is also from 2011
  • Minipreps of Cas9 and GFP storage vector cultures- nanodrop notes
  • Tube Concentration (ng/uL) 260/280 260/230
    Cas9 1:2 from 8/5 52 1.97 1.90
    Cas9 1:3 from 8/1 241 1.90 2.30
    Cas9 1:3 from 8/4 152 1.90 2.22
    GFP 1 storage in pSB1T3 74 1.87 1.44
    GFP 2 storage in pSB1T3 / reblank 63/41 1.82/1.96 1.07/1.78
    GFP 3 storage in pSB1T3 9.4 1.73 0..82

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