Team:Northwestern/09 02

Notebook

Friday, September 2nd

Transformation Results:

MIT plasmids transformed.

Figure 1: MIT pX851

Figure 2: MIT pX852

Figure 3: Positive transformation control

Tasks:

Michelle

  • Miniprepped Cas9 and gRNA template plasmid
    • Cas9 tube 1: 231.7 ng/uL, 260/280: 1.89, 260/230: 2.16
    • Cas9 tube 2: 358.9 ng/uL, 260/280: 1.88, 260/230: 2.30
    • gRNA tube 1: 174.7 ng/uL, 260/280: 1.91, 260/230: 2.26
    • gRNA tube 2: 235.4 ng/uL, 260/280: 1.90, 260/230: 2.25
    • gRNA tube 3: 223.8 ng/uL, 260/280: 1.90, 260/230: 2.23
  • Made Tet+Cam plates:
    • 230 mL LB + 3.4510 g Bacto Agar

Sam

  • Worked on Auburn Gresham plan
  • Added some website content
  • Edited adult pamphlet

Sara

  • Worked on lab notebook
  • Ran a gel on the Cas9 insert with the added restriction sites and on the Cas9-linearized for SS with the extra homology
    • 3 uL SybrSafe
    • Ladder, Cas9-1, Cas9-1, Cas9-2, Cas9-2, space, Cas9SS-1, Cas9SS-1, Cas9SS-2, Cas9SS-2
    • Used 5 uL 1 kb ladder and 4 uL dye

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