Team:Northwestern/09 07

Notebook

Wednesday, September 7th

Transformation Results:

No visible colonies on our Cas9 ligation. The gel screen showed a faint band of the correct size, so we should have seen colonies. We suspect that our transformation efficiency is low.

Figure 1: Cas9 ligation

Figure 2: Positive Gibson control

Figure 3: Transformation positive control

Figure 4: Cas9+ SS Gibson

 

Tasks:

Michelle

  • Ran gel screen on Cas9 restriction digest
    • Really faint bands
    • Ligation had 3 bands, were really hard to see
      • pSB1C3 size, Cas9 size, and one above that

Sam

  • Worked on pamphlet

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