Team:Northwestern/09 17

Notebook

Saturday, September 17th

Tasks:

Sam

  • Gel screen of pET28a
    • 5 μL SybrSafe (Mistake. Should have added 3 μL.)
    • 6 μL 1kb ladder + 3 μL 6X purple loading dye
    • In each sample, 1 μL 6X purple loading dye + 5 μL DNA
    • Size of pet28a: 5369 bases

    • PCR purification of pET28a: Conc: 120.3 ng/μL, 260/280: 1.87, 260/230: 1.83

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