Wednesday, October 5th
Tasks:
Jordan
- Grow up cultures to innoculate Western blot cultures (2 mL)
- JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
- BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9
- Overnight cultures for pSB1C3-gRNA-template & pSB1C3-gRNA-guide (Top10)
Paul
- Send pSB1C3-gRNA-template & pSB1C3-gRNA-guide for sequencing
Jordan
- Grow up cultures to innoculate Western blot cultures (2 mL)
- JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
- BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9
- Overnight cultures for pSB1C3-gRNA-template & pSB1C3-gRNA-guide (Top10)
Paul
- Send pSB1C3-gRNA-template & pSB1C3-gRNA-guide for sequencing
