Team:Northwestern/10 06

Notebook

Thursday, October 6th

Tasks:

Jordan

  • Grow up cultures Western blot cultures to OD=0.6 (15-25 mL)
  • JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
  • BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9 (15 mL)
  • Periplasm procedure on all Western blot cultures
  • Clean out fridge

Sam & Tasfia

  • OMV purification of JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP

Tasfia, Shu, & Sara

  • Start Western blot procedure (run protein gel, load nitrocellulose membrane, start blocking overnight)

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