Thursday, October 6th
Tasks:
Jordan
- Grow up cultures Western blot cultures to OD=0.6 (15-25 mL)
- JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
- BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9 (15 mL)
- Periplasm procedure on all Western blot cultures
- Clean out fridge
Sam & Tasfia
- OMV purification of JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
Tasfia, Shu, & Sara
- Start Western blot procedure (run protein gel, load nitrocellulose membrane, start blocking overnight)
Jordan
- Grow up cultures Western blot cultures to OD=0.6 (15-25 mL)
- JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
- BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9 (15 mL)
- Periplasm procedure on all Western blot cultures
- Clean out fridge
Sam & Tasfia
- OMV purification of JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
Tasfia, Shu, & Sara
- Start Western blot procedure (run protein gel, load nitrocellulose membrane, start blocking overnight)
