Team:Northwestern/gibson

Protocols

Gibson Assembly

  • Master Mix Protocol
    1. In a 2:1 insert to backbone ratio, add 50 ng of the backbone
    2. Add inserts
    3. Incubate at 50 degrees for 1 hr
  • To calculate how much of each insert to add, do this:
    • Insert mass in ng = (Backbone in ng) * (Insert length/Backbone length) * 2:1