Team:Shenzhen SFLS/Collaborations

Collaborations

CollaborationWe got a lot through our iGEM journey, and one of the best and most valuable among them was our cooperation with other iGEM team. In this win-win process, we exchanged our ideas, inspired creativity, and even helped each other to run experiments.

Collaboration With iGEM Team BGIC China
Team BGIC China>>Us

Team BGIC China were working on a programmable and open-source Next Generation Bio-Chip (NGBC) which allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions. They based their NGBC on E. coli S30 Extract System for Circular DNA(Promega), the same with ours. They also utilized enzyme b-galactosidase (LacZ) as one of their reporter gene. So they helped us to test whether S30 system was qualified as a platform for translation of enzyme b-galactosidase (LacZ). Here is their result.



Figure: The blue liquid in the small eppendorf is the S30 system with gene circuit coding enzyme b-galactosidase (LacZ) and its substrates, X-gal, after hours of reaction.

It qualitatively proved that our S30 cell-free system works well.

We also tested this with a different reporter gene, luciferase. Check our results in the “Proof of Concept” section of our wiki.



SFLS>>Team BGIC China We helped Team BGIC China by constructing plasmid pfadr for them. Plasmid pfadr is already in the parts collection and is modified to be responsive to fatty acid. Here are some of the results we got in the process.


Figure 1: the Nanodrop results of plasmid extraction

Figure 2: the PCR result



We have really close relationship with Team BGIC China, because we share the same laboratory with them and received training on the cloning cycled together. We also shared a great part of cloning materials in the beginning of our experiment because our materials haven’t arrived yet. We always had great communication about our projects and on our testing experiments, since our projects are based on the same technology platform-cell-free system.

We have really close relationship with Team BGIC China, because we share the same laboratory with them and received training on the cloning cycled together. We also shared a great part of cloning materials in the beginning of our experiment because our materials haven’t arrived yet. We always had great communication about our projects and on our testing experiments, since our projects are based on the same technology platform-cell-free system.