Team:Toronto/Notebook-w02-wed
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Week 2: May 25, 2016
Wednesday, 5/25
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Finalizing the wet lab budget and proposal abstract:
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"By creating a synthetic biological sensor, we can use it as a cheap, environmentally friendly and easily scalable for detecting gold. We will build cellulose paper with GolS, a transcriptional activator that can express the reporter gene LacZ in presence of gold. The activity of the LacZ can be visualized by a colour change on the paper to purple. This device will allow for a quick, easy and affordable method of detecting gold in soil and water samples. We will also create a mobile app that will use colourimetric analysis to determine the concentration of gold in the sample via the built-in smart phone camera. Along with the mobile app, we intend on designing a pipeline to discover novel gene clusters that are homologous in function to gold resistance and gold accumulation genes to discover new genes and operons that can be used as a green approach to the mining industry. The biosensing aspects can work to replace the existing gold detection methods in the mining industry as an affordable and green synthetic biology approach."
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Proposed/estimated wet lab budget: https://docs.google.com/spreadsheets/d/1MPdm2mD6M06dT9CbcYPBbBYGa-shz_mLt-IwD8b6O6g/edit?usp=sharing
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Predicting the 3D structure of GolS and its variants (https://drive.google.com/folderview?id=0BwhdtcohMX_ocUNNLV9XenlSYTg&usp=sharing)
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Decided to include the His tags on the C terminus with a TEV linker.
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Follow up with Christian Euler regarding the Anderson promoter library
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Asking Christian Euler to become our alternate graduate advisor (he agreed)
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Kayla and Naveen will be gone June 23 - July 11
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Sent out emails to Dr. Madahaven, Dr. Dias, Marc Fume, Dennis and Jordan from OG
For tomorrow:
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Identify best promoter among pgolTS, pgolB and pges
(pgolB: cttgaccttccaacactggcaaggtccagactggcaa)
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Design plasmids using TetO-GFP and TetO-RFP in place of pgolB-LacZ and TetO-golS.
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Decide which golS modification to use (up to two mods, but please rank them in order of favourability)
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Follow up with Christian regarding His-tag
