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Week 4: June 7 2016

Tuesday, 6/7

Members Present: Kat, Hamed, Bohdan, Mark, Celine, Karim

LAB:

Morning:

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Got trained + washed the remainder of glassware in WB19

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[Bohdan, Hamed, Kat] Got trained on the large centrifuge in WB19

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Compiled remainder of SDS and printed out a few at ECS

Afternoon:

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Obtained permission form Susie to use the 95% EtOH from the chemical room in order to make our CAM stocks and 75% EtOH wash bottles [calculations in lab notebook]

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Made 2x75% EtOH (400mL). There are tick marks on the washbottles for ease of making in the future

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Made >30 ~1mL aliquots of 34mg/ml Chloramphenicol (CAM). These were stored in Yuki. Remainder 95% EtOH was put in the flammables cabinet.

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created in silico primers for regular linear backbone

Administrative:

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Verified Medstore account

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Found out that we need to have some form signed by Dr Mahadevan and Adele in order for us to use the credit card. (Adele has signed already, Dr Mahadevan TBD)

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Purchased nitrile gloves (S,M,L) , kimwipes, PCR microtube 0.5mL, 15mL falcon tubes, P20+P200+P1000 tips, and 1.7mL microcentrifuge tubes.

TO DO:

For the next day:

LAB TEAM:

Make LB & LB + CAM plates, store at 4C

Obtain some E.coli DH10B from Naveen (if possible) and grow out in 2 X 50mL of fresh LB in the orbital shaker (either do this first thing in the morning/las thing in the evening)

*Read all MSDS and go through all protocols for this week* ~ you may be tested at any point.

Refer to the adjusted schedule below.

Potentially help out with lab manager stuff.

LAB MANAGERS:

Order:

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Syringes for 0.2uM filter tips

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***PAPER TOWELS**

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SOB powder (we didn't bother with the SOC protocol)

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Scupulas (3-4)

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Stirring rod (only need one)

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Sharpies, pens (loads - bookstore)

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Lock for communal locker (bookstore)

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Label maker

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Look into the price of a scale

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Disposable sterile inoculation loops

Ask Susie for an ethanol burner for 403.

Autoclave:

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LB , 2X LB + Agar

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Pipette tips

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Microcentrifuge tubes (in beaker)

Finish printing out SDS + protocols

Organize the lab, label everything

Inventory the fridges

Adjusted Schedule

	A	B	C	D
1	Tuesday	Wednesday	Thursday	Friday
2	- Antibiotic Stocks	Autoclave media, make plates	- Competent cells	-Transformation efficiency
3	- Media (SOC, LB, LB + Agar) [to be autoclaved]	- Grow out cells	-Transformation efficiency (if time)	- Miniprep
4		- Glycerol stocks		- Nanodrop

Table1

Gmail correspondence:

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E-mail to susie requesting access to downstairs autoclave room

Meetings/Notes:

References:

Team:Toronto/Notebook-w04-tue

Week 4: June 7 2016