Thursday June 23, 2016
Members Present: Marc, Andrea, Celine, Davesh, Karim, Kat
- Transformation did not work - the colony counts are not consistent across different concentrations and duplicates. As there are no controls (ie, with different batches of competent cells, or the cells plated on just LB) it is difficult to assess wether the issue is with the competency of the cells, the transformation, or the viability of the cells themselves. Therefore another attempt with controls was done for the following day.
- Made LB + CAM Plates
- Miniprepped RFP:
- Transformation with the following conditions (including controls):
Gel electrophoresis of both EcoRI and PstI digested RFP plasmid, with and without DpnI. Including a 3kb ladder and miniprepped RFP.
Concluded that our restriction enzymes work appropriately.
For the next day:
If transformation works:
If transformation doesn't:
- make more rubidium chloride competent cells