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Tuesday June 21, 2016

Tuesday, 6/21

Members Present: Marc, Hamed, Kat, Karim, Bohdan, Celine

LAB:

Morning:

- Creating Buffers for Rubidium Chloride Competent Cells protocol

- Culturing cells to reach optimal density to be used in Rubidium Chloride Competent Cells protocol

Afternoon:

- Preparing the competent cells

- Flash-freezing the cells using liquiod nitrogen

Administrative:

TO DO:

For the next day:

LAB TEAM:

- Transformation using the Rubidium Chloride Competent Cells

- Restriction enzyme digest (and ligation?)

LAB MANAGERS:

Gmail correspondence:

Meetings/Notes:

References:

Team:Toronto/Notebook-w06-tue