Team:Toronto/Notebook-w08-thu
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Thursday July 7th, 2016
Thursday, 7/7
Members Present: Karim, Marc, Kat, Caline, Bohdan, Andrea
LAB:
Morning:
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PCR amplified:
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Long Linear plasmid GolS
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Long Linear plasmid GolS P118A
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Short Linear TetO-mCherry
Note that these were done together because their extension temperatures are similar due to their similar size (~990bp)
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Restriction enzyme digest of:
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Long Linear plasmid GolS
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Long Linear plasmid GolS P118A
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Short Linear TetO-mCherry
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O5/O2
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72.4C Backbone
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Ligation of:
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Long Linear plasmid GolS
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Long Linear plasmid GolS P118A
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Short Linear TetO-mCherry
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Glycerol Stocks of BL21 cultures (both LB and 2xYPTG
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Transformation with ligated constructs.
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Run Gel:
GEL: Lane 1: PCR amplified short linear mCherry Lane 2: Digested Short linear mCherry. Lane 3: PCR amplified Long Linear GolS Lane 4: Digested Long Linear GolS Lane 5: PCR amplified Long Linear Gols P118A Lane 6: 2-log ladder Lane 7: DIgested Long Linear GolS P118A Lane 10: Digested pSB1C3 plasmid. Lane 11: Miniprepped O2 lane 12: Miniprepped O5 Lane 12: Single RE digested O5/O2 Lane 13: Double RE digested O5/O3 (sorry about the labelling am tired)
TO DO:
For the next day:
LAB MANAGERS: pick up package from Phill (CPRG)
