Wednesday July 6, 2016
Members Present: DK, Hamed, Kat, Cathy, Alex, Tam
- Using Vacufuge to create concentrated RFP plasmids (for training purposes)
- Growing out the cultures further in preparation for mini-prep
Miniprep using our new NEB monarch kit sample. We miniprepped RFP, and our O2 and O5 overnight cultures.
Nanodrop of the miniprepped samples.
Because O2/O5 sample labelling was confused in the first round of miniprep, the samples were miniprepped again:
Ran gel of our miniprepped O2, O5, and RFP (these are the properly labelled variants). Also included digested O2 and O5, vacufuged and concentrated RFP, and our previous ligation attempt, L1.
Obtained BL21 from Christian -> made 5ml cultures in 2xYPTG and in LB, and also streaked out a LB plate.
Spoke to the NEB guy. Obtained some ladder samples as well as a few miniprep and gel extraction sample kits.
Digest the O2 with EcoRI and with both EcoRI and PstI to figure out what's up with the weird size of our ligated constructs (should be ~2470bp)
Run PCR amplification with the rest of our IDT constructs (2 step)
Run a gel with the questionable O2 and O5.