Team:Toronto/Notebook-w10-mon

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Monday - July 18

Monday, 7/18
Members Present:
LAB:
Morning:
Counted the colonies for the transformed cultures (using ligated products)
L1 digested.jpg
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L1 undigested.jpg
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L2.jpg
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L3.jpg
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L4.jpg
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L5.jpg
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no plasmid control.jpg
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Conducted PCR on all constructs
Afternoon:
Created a small BL21 liquid culture
Created 5mL cultures for miniprep for the important colonies (see table under Tuesday, July 19)
Ran two gels on all the PCR products:
Gel1.jpg
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Lane 1: Empty Lane 2: TetO_LacZ Lane 3: TetO_mCherry Lane 4: Ladder Lane 5: pgolB_LAcZ Lane 6: Long GolS P118A Lane 7: Short TetO GolS P118A
Gel2.jpg
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Lane 1: Empty Lane 2: Long GolS Lane 3: Short TetO_GolS Lane 4: Short PrcnA_LacZ Lane 5: Ladder Lane 6: Short TetO_RncR Lane 7: BB from Amil CP
Administrative:
Items Purchased:
A
B
C
D
E
F
1
NameSKUUnit PriceQuantityTotal (incl. tax)Status
2
Alliance Nitrile Gloves (S)211-62057.5325.43Available
3
Alliance Nitrile Gloves (M)211-62067.5325.43Available
4
Grand Total50.85
Table2

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