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Monday - July 18

Monday, 7/18

Members Present:

LAB:

Morning:

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Counted the colonies for the transformed cultures (using ligated products)

L1 digested.jpg

L1 undigested.jpg

L2.jpg

L3.jpg

L4.jpg

L5.jpg

no plasmid control.jpg

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Conducted PCR on all constructs

Afternoon:

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Created a small BL21 liquid culture

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Created 5mL cultures for miniprep for the important colonies (see table under Tuesday, July 19)

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Ran two gels on all the PCR products:

Gel1.jpg

Lane 1: Empty Lane 2: TetO_LacZ Lane 3: TetO_mCherry Lane 4: Ladder Lane 5: pgolB_LAcZ Lane 6: Long GolS P118A Lane 7: Short TetO GolS P118A

Gel2.jpg

Lane 1: Empty Lane 2: Long GolS Lane 3: Short TetO_GolS Lane 4: Short PrcnA_LacZ Lane 5: Ladder Lane 6: Short TetO_RncR Lane 7: BB from Amil CP

Administrative:

Items Purchased:

	A	B	C	D	E	F
1	Name	SKU	Unit Price	Quantity	Total (incl. tax)	Status
2	Alliance Nitrile Gloves (S)	211-6205	7.5	3	25.43	Available
3	Alliance Nitrile Gloves (M)	211-6206	7.5	3	25.43	Available
4				Grand Total	50.85

Table2

Team:Toronto/Notebook-w10-mon