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Wednesday, July 20

Wednesday, 7/20

Members Present: Tam, DK, Hamed, Kat, Cathy

LAB:

Morning:

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PCR amplification of Long GolS P118A

Afternoon:

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Gel Extraction of PCR-amplified Backbone from RFP (the following are the nanodrop results; there are currently 10ul in each tube):

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Ran a gel on gradient PCR done on Long GolS P118A:

Lane 1: 1A Lane 2: 1B Lane 3: 1C Lane 4: 1D Lane 5: 1E Lane 8: DNA Ladder Lane 9: 2A Lane 10: 2B Lane 11: 2C Lane 12: 2D Lane13: 2E

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Started an overnight culture of BL21 for larger cultures tomorrow

Team:Toronto/Notebook-w10-wed