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Saturday, July 30

Tuesday, 8/2

Members Present: Alex, Cathy

LAB:

Afternoon:

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Counted cells on transformed plates from Friday, July 29:

	A	B
1	Plate	Colonies count
2	HISR+pCOLA (1)	38
3	HISR+pCOLA (2)	44
4	Kana Negative	0
5	Cam competence negative	0
6	Cam water negative	0
7	BB DPN1 (1)	14
8	BB DPN1 (2)	7
9	BB Digested (1)	0
10	BB Digested (2)	21
11	BB Undigested (1)	0
12	BB Undigested (2)	0

Transformed Plate Count

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All cells were white

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NOTE: BB Undigested (1) appeared to be wet (not condensation wet but actually wet wet)

IMG_20160730_143436.jpg

HISR+pCOLA (1) and (2), respectively having 38 and 44 cells as well as Kanamycin negative (nothing on the plate) having no colonies

IMG_20160730_143442.jpg

Cam water negative and competence negative, both with no colonies

IMG_20160730_143447.jpg

BB DPN1 (1) and (2) on Cam plates. Respectively having 14 and 7 colonies

IMG_20160730_143452.jpg

BB Digested (1) and (2) on Cam plates. Respectively having 0 and 21 colonies.

IMG_20160730_143456.jpg

BB Undigested (1) and (2) on Cam plates. Both having no colonies grown. BB Undigested (1) appear to be wet (not condensation wet but actually wet wet)

TO DO:

For the next day:

LAB TEAM:

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Transform the plasmids

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