Team:Toronto/Notebook-w12-thu

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Thursday, August 4th

Thursday, 8/4
Members Present: Karim, Marc, Celine, Kat, Hamed, Bohdan
LAB:
Morning:
Ran a gel on undigested miniprepped plasmid, in hopes of determiining wether or not the gel from the previous day corresponded to one plasmid digested or two separate plasmids:
Gel 1.jpg
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Gel 2.jpg
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PCR amplified backbone, short p118A, short GolS, pgolB lacZ
Afternoon:
Transformation with CCE cells (0.5pg, 10pg, 50pg of RFP), and with CCD of LacZ constructs
Gel of PCR amplified constructs:
gel 3.jpg
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gel 4.jpg
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Administrative:
Purchases:
A
B
C
D
E
F
1
NameSKUUnit PriceQuantityTotal (incl. tax)Status
2
P100070.7622.52 (250/pack)5.65available
3
1.7mL microcentrifuge tubesLMCT1.7B19121.47available
4
Monarch DNA Gel Extraction Kit - 50 prepsT1020S108.421122.51Next Day Delivery
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TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
Pick up Monarch DNA Gel Extraction Kit - 50 preps
Gmail correspondence:
Meetings/Notes:
References: