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Tuesday, August 2

Tuesday, 8/2

Members Present:

LAB:

Morning:

●

Completed day 2 of the S30 protocol (after 12 weeks). Flash froze the extracts and stored them at -80 (both normal and runoff reactions)

●

Made RF1 and RF2 for rubidium chloride competent cells

Afternoon:

●

Made ovenight cultures of BL21 and DH10B for competent cells

●

Made ovenight cultures of the colonies that appeared following transformation on friday.

Administrative:

TO DO:

For the next day:

LAB TEAM:

LAB MANAGERS:

Gmail correspondence:

Meetings/Notes:

References:

Team:Toronto/Notebook-w12-tue