Team:Toronto/Notebook-w14-mon

console.js:26

Monday, August 15

Monday, 8/15
Members Present: Alex, Cathy, Hamed, Marc, Zarifah
LAB:
Morning:
Contamination was detected in all cultures which had used CCF (including negative control for transformation efficiency test)
RF1 and RF2 Buffers were prepared to create a new batch of competent cells
Previous day transformation was prepared in liquid solution for overnight growth
Afternoon:
Begin making RbCl competent cells (used CCC cells)
First measurements at 4:40 was around 0.011, around 7:30 was around 0.120
Streaked the 0.120 CCC cells onto the following plates:
2 Lb plates (keep one for later reference)
1 Lb+CAM (control)
NOTE: The Lb plates appeared unusually liquid to the extent they were 'dripping'
Also streaked CCF cells to make sure they not contaminated on CCF cells
75ul instead of 50ul was added
Administrative:
Alex contacted the following people:
Dr. Mahadevan regarding meeting and potential extension
Keith regarding meeting
oGEM regarding Skype meeting for Geonomics in the Park
Meeting notes from the weekly meeting can be found here:
https://drive.google.com/folderview?id=0B6Z4m6vl-WRuUVNFMEU1UTB5Q0U&usp=sharing
TO DO:
For the next day:
LAB TEAM:
Grown overnight cultures of the previous day streaked cells for next day making of RbCl competent cells
Miniprep and visualize the overnight solutions of last weeks transformation