Team:Toronto/Notebook-w14-thu
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Thursday, August 18
Thursday, 8/18
Members Present: Alexander Sullivan, Zarifah Omar Ali, Celine Zhang, Karim Sarif, Cathy Cao
LAB:
Morning:
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Made more Lb+Cam plates
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Inventory was completed and can be found here: https://docs.google.com/spreadsheets/d/1D0URRjnUUPMO5bKTFUPoA443ZDUa0SG6bsjcdh6AF4A/edit?usp=sharing
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Chemical room was cleaned
Afternoon:
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Plates from previous day was checked (Cell counted) and a liquid overnight culture was prepared from it
A | B | |
1 | Plate | Cell count |
2 | CCF | 1 |
3 | CCG | 0 |
4 | R1 | 23 |
5 | SG1 | 14 |
6 | SP1 | 9 |
7 | C1 | 9 |
8 | P1 | 14 |
9 | G1 | 0 |
10 | Control | 0 |
Table1
All colonies appeared to be white. P1 were very tiny. For understanding notation:
CCF and CCG are competent cells. R is pgolB_LacZ. SG is Short_GolS. SP is Short P118A. C is mCherry. P is Long_P118A. G is Long_GolS.
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Photos:
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CCG competent cells were tested for competency
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Plates were made for 0.5pg/ul, 5pg/ul, 10pg/ul, 20pg/ul and 50pg/ul of iGEM RFP competency testing on Lb+Cam plates. Three controls were made: CCG cells with no DNA transformed in on Lb+Cam plate and a Lb+Agar (no Cam) plate as well as just water on Lb+Cam plate (negative control)
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Protocol reference: iGEM Competent Cell Test Kit
Administrative:
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Meeting with Keith Pardee was organized for 1pm on Monday, August 22nd
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P&P is asking for anyone who wish to volunteer at the summer camp, please message Alexander Sullivan if you are interested
