Team:Toronto/Notebook-w16-tue

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Tuesday - August 30, 2016

Tuesday, 8/30
Members Present: Bohdan, Kat, Celine, Hamed, Karim, Marc.
LAB:
Morning:
A
B
C
D
E
F
G
H
I
1
C1P2R2G2SG1SP2
2
Miniprep Concentration17.913.920.234.644.544.5
3
Nuclease-free water0005.448.010.12
4
DNA17171711.568.9916.88
5
Cutsmart222222
6
Enzyme111111
7
Total volume20ul20ul20ul20ul20ul20ul
8
Final DNA Conc.15.21515.8117.17202020
Table1
Afternoon:
Gel extracted the the remainder of the constructs
RE digested the extracted constructs
Ran a gel on the extracted constructs:
gel1.jpg
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Lane 1: SP construct (short GolS P118A) Lane 2: SG (Short GolS) Lane 3: Long GolS P118A Lane 4: 2-log ladder Lane 5: reporter Lane 6: Long GolS Lane 7: mCherry
Lane 1: LacZ high band Lane 2: 2-log ladder Lane 3: LacZ low band
gel2.jpg
thumbnail
Made O/N cultures of the constructs that were unsuccessfully extracted.
Transformed with miniprepped plasmid DNA from the stock initial assembled constructs.
Administrative:
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
Gmail correspondence:
Meetings/Notes:
References: