Team:Toronto/Notebook-w18-fri
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Friday, September 16
Sunday, 9/18
LAB:
Afternoon:
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Checked on the 96-flat-well plates after 48 hours based off of the experiment taken place on Wednesday, September 14 (absorbance results can be see on this file: https://drive.google.com/file/d/0B6Z4m6vl-WRuZm1jdHhHM1d4em8/view?usp=sharing )
(R=Row, C=Colume):
RAC1: mRFP1 (faint) - There is no distinct colour present in here as there is no CPRG present (or LB for it to grow plentifully)
RAC2: mCherry (faint) - There is no distinct colour present in here as there is no CPRG present (or LB for it to grow plentifully)
RAC3: LacZ1+1mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). NOTE: The clear center could be due to it drying up as this is 48 hours after initial test
RAC4: LacZ2+1mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here)
RAC5: LacZ2+1mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). NOTE: The clear center could be due to it drying up as this is 48 hours after initial test
RAC6: pgolB_LacZ+1mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here)
RBC1: mRFP1 (faint) - There is no distinct colour present in here as there is no CPRG present (or LB for it to grow plentifully)
RBC2: mCherry (faint) - There is no distinct colour present in here as there is no CPRG present (or LB for it to grow plentifully)
RBC3: LacZ1+5mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). Darker than the row above (RA) due to it having more CPRG in the well. NOTE: The clear center could be due to it drying up as this is 48 hours after initial test
RBC4: LacZ2+5mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). Darker than the row above (RA) due to it having more CPRG in the well. NOTE The white dots on top of the well is a thumb smudge.
RBC5: LacZ2+5mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). Darker than the row above (RA) due to it having more CPRG in the well. NOTE The white dots on top of the well is a thumb smudge.
RBC6: pgolB_LacZ+5mM CPRG - The LacZ that is being expressed is able to cleave the yellow CPRG into a purple subtrate (as seen here). Darker than the row above (RA) due to it having more CPRG in the well. NOTE The white dots on top of the well is a thumb smudge.
RCC1: Lb - No colour visible here as only a small amount of Lb was added. Nothing should be visible here as it can be an indication for base contamination
RCC3: LacZ1 - This well is not distincty yellow or purple as no CPRG was added. The yellow-ness around the bottom of the well is due to the bacteria.
RCC4: LacZ2 - This well is not distincty yellow or purple as no CPRG was added. The yellow-ness around the bottom of the well is due to the bacteria.
RCC5: LacZ3 - This well is not distincty yellow or purple as no CPRG was added. The yellow-ness around the bottom of the well is due to the bacteria.
RCC6: pgolB_LacZ - This well is not distincty yellow or purple as no CPRG was added. The yellow-ness around the bottom of the well is due to the bacteria.
RHC3: DH10B+1mM CPRG - A CPRG negative control where only E. coli K12 DH10β was added to a solution of 1mM CPRG. This was used to keep the sample as yellow as possible though now it is a bit faint as it has begun to try up.
RHC5: BL21+1mM CPRG - A CPRG positive where BL21 (is not LacZ deficient) was added to 1mM of CPRG. This was used to keep the sample as purple as possible though now it is a bit faint as it has begun to try up.
A | B | C | D | E | F | G | |
1 | 1 | 2 | 3 | 4 | 5 | 6 | |
2 | A | mRFP1 (faint) | mCherry(faint) | LacZ1+1mM CPRG | LacZ2+1mM CPRG | LacZ2+1mM CPRG | pgolB_LacZ+1mM CPRG |
3 | B | mRFP2(faint) | mCherry(faint) | LacZ1 +5mM CPRG | LacZ2+5mM CPRG | LacZ2+5mM CPRG | pgolB_LacZ+5mM CPRG |
4 | C | LB | LacZ1 | LacZ2 | LacZ3 | pgolB_LacZ | |
5 | H | DH10B+1mM CPRG | BL21+1mM CPRG | ||||
Table1
Administrative:
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Meet with OG regarding:
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Genomics in the Park
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Attendee: Ben (P&P), Alexander Sullivan, Jordan (OG)
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Ben (University of Toronto) and Ayan (Waterloo University) are confimed to go
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Jordan from OG will be designing the poster
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We have to design the broucher
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Synthetic Biology Network Platform (P&P project)
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Attendee: Alexander Sullivan, Shivali (P&P), Fahim (Dry lab), Sana (OG), three people from ORION
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We got the server
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1 year on server, renew in one year if we wish to continue
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OG wants long-term plan
TO DO:
For the next day:
LAB TEAM: (For Cathy Cao, Alexander Sullivan, Katariina Jaenes, Hamed Tinafar, Celine Zhang, Karim Sarif)
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Culture cells and miniprep
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Miniprep for sequencing and Concordia iGEM collaboration
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Start AuCl assaying if we have new plates and the AuCl are in
LAB MANAGERS: (For Zarifah Omar Ali, Bohdan Lukach)
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Dishwashing
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Pick up IDT orders that have arrived at Phil's (WB basement)
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Autoclaving pipette tips and anything else needed
