Polychlorinated biphenyls (PCB’s) were infamous in the past, and they are still a threat today. PCB’s are organic compounds that were used extensively in various electrical industries in the past, specifically as a coolant. However, the production and usage was banned in the United States of America in 1979, and in the Stockholm Convention on Persistent Organic Pollutants in 2001. This action was in response to the discovery that PCB’s are non-biodegradable and cause serious neurological, reproductive and immune disorders in both humans and animals.
PCB’s are still present in many water sources around the world, and they may cause problems through bioaccumulation. The EPA set the standard that more than 0.0005ppm of PCB is bad for the environment and human health. Clean water is becoming one of the most important and dwindling resources globally.
Hence, the UNH iGEM Team aims to build a sensitive PCB biosensor that detects the base biphenyl structure in water sources. Bacteria like Pseudomonas pseuoalcaligenes, Acidovorax sp., Burkholderia, Luteibacter, Williamsia, etc. can degrade PCB’s, and the enzymes that do so are coded by the bph operon. We want to build a system based on the bph operon, such that production of an intermediate of the PCB degradation pathway will give a fluorescent output. Previous iGem teams have attempted to design similar biosensors based on the bph operon, but have struggled to obtain positive results. Teams such as Evry and Paris-Saclay had been unable to achieve positive results, and the current testing methods are unable to detect to the EPA’s standards for PCB. Our team aims to develop a more accurate and precise biosensing tool using bacterium to identify lower concentrations of PCB than are presently available.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to
T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)