Team:UPMC-Paris/Material

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Kits

1. .Monarch Plasmid Miniprep Kit from NEB Ref: T1010S
2. .Monarch PCR & DNA Cleanup Kit (5ug) Ref: T1030S
3. .Phusion High-Fidelity PCR Kit from NEB Ref: E0553S
4. .QiAquick Gel Extraction Kit from Qiagen Ref: 28704
5. .QiAquick PCR Purification Kit from Qiagen Ref: 28104

Bacterial Strains

You can find our bacteria here!

Media and chemicals

.LB Broth-Miller from Difco, Ref: 244620
.Bacto Yeast Extract from Difco, Ref: 212750
.Quick-Load 1 kb DNA Ladder from NEB, Ref: N0468S
.Formic Acid Free Acid from Sigma-Aldrich, Ref: F0507-100ML-D
.Manganese(II) Sulfate Monohydrate from Sigma-Aldrich, Ref: M7634-100G-D
.Zinc Sulfate Hepthydrate from Sigma-Aldrich, Ref: Z4750-100G-D
.Ferric Chloride Hexahydrate from Sigma-Aldrich, Ref: F2877-100G-D
.Thiamine Hydrochloride from Sigma-Aldrich, Ref: T4625-5G
.Chelex 100 Sodium Form (Chelating Resin) from Sigma-Aldrich, Ref: C7901-25G
.Nicotinic Acid 99% from Grosseron, Ref: A12683.30
.Phusion High-Fidelity PCR Master Mix with HF Buffer from Thermo Fisher Scientific, Ref: F531S

Following chemicals were provided by our supervisors:

Ammonium Sulfate (powder)

TrisHCl 1M pH 7.8

Tri Sodium Citrate (powder)

EGTA 0,1M pH 8.2

Calcium Chloride (powder)

Casamino Acid 1%

SDS 20%

TE pH 8

Magnesium Sulphate Trihydrate (powder)

Triton X100

Agarose

Agar Agar

MiliQ Water

Sodium Hydroxide (powder)

Sodium Chloride (powder)

Sulfate 2n (powder)

Magnesium Sulfate (powder)

Potassium Acetate (powder)

Tris Base (powder)

EDTA (powder)

EGTA (powder)

Potassium phosphate monobasic (powder)

Glucose (powder)

Enzymes

.T4 DNA Ligase from Sigma-Aldrich, Ref: 10481220001
.Pvu II, recombinant from NEB, Ref: R0151S
.Avr II, recombinant from NEB, Ref: R0174S
.Pac I, recombinant from NEB, Ref: R0547S
.Psc I from Thermo Fisher Scientific, Ref: ER1871
.Kpn I from Thermo FIsher Scientific, Ref: ER0521
Following enzymes were provided by our supervisors:
.Alkaline Phosphatase
.EcoR I
.Xba I
.Spe I
.Sac I
.Kpn I
.BamH I
.Sal I
.Xho I

Antibiotics

.Cloramphenicol from Sigma-Aldrich, Ref: C0378-5G
.Kanamycine sulfate from Termo Fisher Scientific, Ref: 11815024
.Ampiciline provided by our supervisors

DNA and Plasmids

Our PCR primers were synthesized by Eurofins
Our Biobricks fragments were synthesized by IDT
We use the plasmid pSB1C3 provided in the iGEM Welcome Kit for fragment and biobrick amplification
We use the plasmid pBS1C provided by Addgene (plasmid #55168) for biobrick integration in B. subtilis.