Our project does not have a finished product that is ready to be deployed in the field. However, we felt that we have accomplished a great many parts of our project and we want to show you what we did.
Sensing Varroa in Infected Beehives
We demonstrated the functionality of the mite-sensing system in beehives. We cannot demonstrate the BeeT product in the field, as this would break the do not release requirement. We also cannot keep a beehive in the lab, for obvious reasons. We tested the guanine and vitamin B12 riboswitches on crushed up hive fragments (Figure 1).
From Figure 1 becomes apparent that the beehive samples cause a different mRFP response on both mite sensing systems. Initially, the mRFP amount is increases more rapidly in samples containing beehive fragments. This suggests that the constructs are indeed able to sense and report guanine and vitamin B12 in contaminated beehives.
BeeT Survival in Beehive Conditions
We simulated the sugar water conditions, BeeT would encounter when applied, in the lab to check for survival. This is shown in Figure 2.
Effectiveness of BeeT on BeecoloniesAdditionally, we modeled the impact of BeeT on bee and mite dynamics under real world weather conditions. We show this in figure 3.
We asked design student Thieu Custers, from the Design Academy Eindhoven, to develop a visual prototype. Below you can find his design and explanation.
See our design page.