Team:William and Mary/Attributions
We've made it a long way since the summer began, and we could not have done it without
the numerous people and organizations that supported us.
First and foremost, we would like to thank our two team advisors, Dr. Margaret Saha, PI, and Dr. Gregory Smith,
Co-PI. For her countless hours of time and unbounded dedication to our cause, we extend our sincerest thanks to
Dr. Saha. We would also like to extend a special thanks to Dr. Smith for his expertise in mathematical modeling
and his willingness to advise our math team. In addition, we offer our thanks to the Vice Provost for Research,
Dr. Dennis Manos, who has provided strong support, financial and intellectual, for iGEM and the BioEngineering
Initiative at William and Mary. We also thank Dr. Eric Bradley for his endless assistance with facilities and
equipment. Finally, we thank Dr. Mark Forsyth, Dr. Helen Murphy, Dr. Bev Sher, and Dr. Doug Young for their
advice on the project.
We would also like to thank the following organizations and offices which have generously provided us with the
financial support necessary to carry out our project, in terms of either supplies or summer stipends.
Office of the Vice Provost for Research and Graduate Studies
William & Mary Dean of Arts & Sciences
William & Mary Roy R. Charles Center
William and Mary Office of Student Leadership Development
EXTREEMS-QED(NSF)
HHMI Undergraduate Science Education Grant
Arnold and Mabel Beckman Foundation
1693 Scholar Program
James Monroe Scholar Program
Donors to our Experiment Page
1. Nielsen, A. A., Segall-Shapiro, T. H., & Voigt, C. A. (2013). Advances in genetic circuit design:
Novel biochemistries, deep part mining, and precision gene expression. Current Opinion in Chemical Biology,
17(6), 878-892. doi:http://dx.doi.org/10.1016/j.cbpa.2013.10.003
2. Sun, Z. Z., Yeung, E., Hayes, C. A., Noireaux, V., & Murray, R. M.Linear DNA for rapid prototyping of
synthetic biological circuits in an escherichia coli based TX-TL cell-free system. ACS Synthetic Biology, (6), 387.
doi:10.1021/sb400131a
3. Lucks, J. B., Qi, L., Whitaker, W. R., & Arkin, A. P. (2008). Toward scalable parts families for predictable
design of biological circuits. Current Opinion in Microbiology, 11(6), 567-573. doi:http://dx.doi.org/10.1016/j.mib.2008.10.002
4. Lou, C., Stanton, B., Chen, Y., Munsky, B., & Voigt, C. A. (2012). Ribozyme-based insulator parts buffer
synthetic circuits from genetic context. Nature Biotechnology, (30), 1137-1142.
5. Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell 146, 105–118 (2011).
6. Brewster, R. C., Weinert, F. M., Garcia, H. G., Song, D., Rydenfelt, M., & Phillips, R. (2014). The transcription factor titration effect dictates level of gene expression. Cell, 156(6), 1312. doi:10.1016/j.cell.2014.02.022
7. Castillo-Hair, S. M., Sexton, J. T., Landry, B. P., Olson, E. J., Igoshin, O. A., & Tabor, J. J. (2016). FlowCal: A user-friendly, open source software tool for automatically converting flow cytometry data from arbitrary to calibrated units. ACS synthetic biology.
8. Daniel, R., Rubens, J. R., Sarpeshkar, R., & Lu, T. K. (2013). Synthetic analog computation in living cells.Nature, 497, 619-623. doi:doi:10.1038/nature12148
9. Milo, R., Phillips, R., & Orme, N. (2015). Cell biology by the numbers. Garland Science
10. Nielsen, A. A., Segall-Shapiro, T. H., & Voigt, C. A. (2013). Advances in genetic circuit design: Novel biochemistries, deep part mining, and precision gene expression. Current Opinion in Chemical Biology, 17(6), 878-892. doi:http://dx.doi.org/10.1016/j.cbpa.2013.10.003
11. Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2014). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182-187. doi:10.1021/sb400089c
12. So LH, Ghosh A, Zong C, Sepu´lveda LA, Segev R, Golding I. General properties of transcriptional time series in Escherichia coli. Nat Genet. 2011 Jun43(6):554-60
13. Torella, J. P., Boehm, C. R., Lienert, F., Chen, J., Way, J. C., & Silver, P. A. (2014). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic Acids Research,42(1), 681-689. doi:doi:10.1093/nar/gkt860
14. Briggs, A. W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., & Church, G. M. (2012). Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic Acids Research, 40(15), e117. http://doi.org/10.1093/nar/gks624
Attributions
Funding Support
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References
