Tsinghua 2012

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Lysozyme hydrolysation of peptidoglycanes leads to cell lysis

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
T7 Promoter Promoter for expression of FimE
LacO lac operon for expression of FimE
Lysozyme hydrolysation of peptidoglycanes leads to cell lysis
FimE invertase that recognizes specific DNA sequences and reverses the sequence
IRL recognition site for FimE
IRR recognition site for FimE