BNU China 2014

Implemented kill switches: 1

Kill Switch 1

Class

Linear line of repressors

Toxins

Name Number Comment
MazF K302033 Coding toxic protein MazF

Antagonists

Name Number Comment
MazE Not used here

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG
LacI K1088018 Coding lacI repressor from E. coli
CI C0051 Coding cI
Promoter 1 J23116 Weak constitutive promoter
Promoter 2 R0010 LacI repressible promoter
Promoter 3 R0051 CI repressible promoter
Double terminator B0015 Double terminator