Tokyo NoKoGen 2010

Implemented kill switches: 3

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
T4 Holin BBa_K112805 Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer
T4 endolysin BBa_K112806 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer

Antagonists

Name Number Comment
Antiholin BBa_K112807 Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria

Topology

Kill Switch topology

Parts

Name Number Comment
AHL receptor lysis BBa_K317039 complete AHL-mediated lysis system

Kill Switch 2

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
T4 Holin BBa_K112805 Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer
T4 endolysin BBa_K112806 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer

Antagonists

Name Number Comment
Antiholin BBa_K112807 Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria

Topology

Kill Switch topology

Parts

Name Number Comment
AHL receptor lysis BBa_K317040 complete IPTG-mediated lysis system

Kill Switch 3

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
T4 Holin BBa_K112805 Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer
T4 endolysin BBa_K112806 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer

Antagonists

Name Number Comment
Antiholin BBa_K112807 Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria

Topology

Kill Switch topology

Parts

Name Number Comment
lux pR BBa_R0062 This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer (3-oxo-hexanoyl-HSL)
luxR repressor/activator BBa_C0062 In complex with HSL and LuxR binds to the Lux promoter
Enterobacteria phage T4 Lysis Device BBa_K112808 This is the lysis device using three genes from enterobacteria phage T4: lysozyme and holin and antiholin