UChile 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
lysis protein

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
pBAD BBa_K206000 promotor induced by arabinose
TetR BBa_C0040 TetR protein which represses pTetR
pTetR BBa_R0040 constitively active promotor which is repressed by TetR
RBS BBa_B0034 ribosome binding site
Ter BBa_B0015 terminator
Lysis gene BBa_K117000 lysis protein - activates endonuclease activity of ColE7 and partial lysis of host cell membrane