UFSCar Brasil 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
KillerRed chromoprotein activated by luminous irradiation - production of reactive oxygen species
Barnase bacterial protein with ribonuclease activity from Bacillus amyloliquefaciens- shows intense lethality

Antagonists

Name Number Comment
Zur BBa_K1620004 zinc uptake regulation protein - binds Zn2+ as a cofactor and negatively controls the promotor Zasp

Topology

Kill Switch topology

Parts

Name Number Comment
Zasp BBa_K1620001 zinc activity sensing promoter element - active in the absense of zinc and Zur-dependent
RBS BBa_B0030
KillerRed chromoprotein activated by luminous irradiation - production of reactive oxygen species
Barnase bacterial protein with ribonuclease activity from Bacillus amyloliquefaciens- shows intense lethality
Ter BBa_B1010 artificial terminator
pCons BBa_J23101 constitive promotor
Zur BBa_K1620004 zinc uptake regulation protein - binds Zn2+ as a cofactor and negatively controls the promotor Zasp
pUspA BBa_K1620000 stress promotor induced under several stress conditions
SmtA BBa_K1620007 Synechococcus metallothionenin A - able to bind heavy metal ions