Bonn 2012

Implemented kill switches: 2

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
ccdB attacks gyrase (a topoisomerase II) which results in the blockade of polymerases.

Antagonists

Name Number Comment
ccdA inhibits the toxic activity by forming a ccdA:ccdB complex

Topology

Kill Switch topology

Parts

Name Number Comment
LOV2 2012 BBa_K820000 contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm).
promoter (lacI regulated) BBa_R0010 In the absence of LacI protein and CAP protein this part promotes transcription.
RBS BBa_B0032 Ribosom binding site
T1 BBa_B0010 T1 from e. coli rrnB
TE BBa_B0012 Transcription terminator for the E.coli RNA polymerase

Kill Switch 2

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
mazF is a sequence-specific ribonuclease from B. subtilis and E. coli.

Antagonists

Name Number Comment
mazE counterbalance mazF

Topology

Kill Switch topology

Parts

Name Number Comment
LOV2 2012 BBa_K820000 contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm).
promoter (lacI regulated) BBa_R0010 In the absence of LacI protein and CAP protein this part promotes transcription.
RBS BBa_B0032 Ribosom binding site
T1 BBa_B0010 T1 from e. coli rrnB
TE BBa_B0012 Transcription terminator for the E.coli RNA polymerase
mazF BBa_K302033 Encodes a stable sequence-specific ribonuclease