Constitutive promoter |
BBa_J23116 |
BBa_J23116 is a constitutive promotor and member of the BBa_J23xxx family promotors. |
RBS.3 (medium) |
BBa_B0032 |
Weak1 RBS based on Ron Weiss thesis. Efficiency of 0.3. |
Double terminator |
BBa_B0015 |
The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar. |
tetracycline repressor from transposon Tn10 (+LVA) |
BBa_C0040 |
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (Part:BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation. |
TetR repressible promoter |
BBa_R0040 |
Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog aTc. |
key3c |
BBa_J23008 |
Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. |
key3d |
BBa_J23009 |
riboregulator for lock3 variants. |
T7 RNA Polymerase with UmuD derived tag |
BBa_K145014 |
T7 RNA Polymerase will start the transcription from a T7 promoter (such as BBa_I712074). It is tagged with a UmuD tag (at the N-terminal) for faster degradation. |
T7 promoter (strong promoter from T7 bacteriophage) |
BBa_I712074 |
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. |
lock3i |
BBa_J23078 |
lock3d derivative with a 5' extension. This is the basic part and J23077 is the RFP reporter. |
LuxI |
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Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone) also known as VAI or N-(beta-ketocaproyl)homoserine lactone or 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-hexanamide an autoinducer molecule which binds to LuxR and thus acts in bioluminescence regulation. |
lacI repressor from E. coli (+LVA) |
BBa_C0012 |
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation therefore promoting transcription. A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part. |
Promoter (lacI regulated and lambda pL hybrid) |
BBa_R0011 |
Inverting regulatory region controlled by LacI (BBa_C0010 and BBa_C0012 and etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:repressed by LacI - the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]). Induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range. |
autoinducer inactivation enzyme from Bacillus that hydrolyzes acetyl homoserine lactone |
BBa_C0060 |
Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged (for untagged version see Part:BBa_C0160. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl-homoserine lactones (AHLs)--quorum sensing autoinducers. |
luxR repressor/activator |
BBa_C0062 |
In complex with HSL the LuxR binds to the Lux promoter and activates transcription from Pr BBa_R0062 and repressing transcription from Pl BBa_R0063. |
Hybrid promoter: HSL-LuxR activated and P22 C2 repressed |
BBa_K145150 |
Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; OR2 and OR1 located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low. |
RBS (Elowitz 1999) |
BBa_B0034 |
RBS based on Elowitz repressilator. |
double terminator (B0012-B0011) |
BBa_B0014 |
The mfold results are annotated with the location of the subparts BBa_B0012 and BBa_B0011. |
ccdB coding region |
BBa_K145151 |
Coding region for the ccdB (control of cell death) gene. This part will cause Top10 cells to die. |
RBS.4 (weaker) |
BBa_B0033 |
Weaker RBS based on Ron Weiss thesis. Strengths relative to BBa_B0030 and BBa_B0031 and BBa_B0032. |
cI repressor from phage 434 (+LVA) |
BBa_C0052 |
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS. |
Promoter (p22 cII regulated) |
BBa_R0053 |
The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein BBa_C0053 binds to it. |
c2 repressor from Salmonella phage P22 (+LVA) |
BBa_C0053 |
The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence BBa_R0053. The sequence contains a LVA tag for faster degradation. |
Standard Promoter (434 cI regulated) |
BBa_R1052 |
The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein BBa_C0052 binds to it. |
cI repressor from phage 434 (no LVA) |
BBa_C0056 |
|
antisense LuxI |
BBa_K145013 |
This part is a RNA that is the complement of luxI (part BBa_C0061). It will bind to the mRNA of luxI and thereby repress the translation of luxI. So in the presence of this antisense luxI the enzym luxI will not be produced. |