METU Turkey 2014

Implemented kill switches: 1

Kill Switch 1

Class

Linearly repressed holin with additional antiholin security

Toxins

Name Number Comment
Holin K124014 Kills the cell
T4 endolysin K112806 Kills the cell

Antagonists

Name Number Comment
T4 Antiholin K515004

Topology

Kill Switch topology

Parts

Name Number Comment
araC-Pbad K808000
TetR+LVA C0040
TetR repressible promoter R0040