Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
T4 Holin |
BBa_K112000 |
membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane |
Cathelicidin LL-37 |
|
antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs |
Topology
Parts
Name |
Number |
Comment |
pCons |
BBa_J23100 |
constitutive promotor |
CymR |
|
cumate-responsive regulator CymR - can bind to T5 cumate operator |
RBS |
|
|
Ter |
|
|
T5 cumate operator |
|
cumate responsive promotor inhibited by CymR |
T4 Holin |
BBa_K112000 |
membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane |
Cathelicidin LL-37 |
|
antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs |