BGU Israel 2013

Implemented kill switches: 2

Kill Switch 1

Class

Repression of holin with multiple linearly arranged repressors

Toxins

Name Number Comment
Holin
Lysozyme K112022

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
arabinose
araC
IPTG
LacI
cI repressor
promoter R0051
P.A.S.E 1 K1223001

Kill Switch 2

Class

Disfunctional essential protein in absense of fulfillment of parallel circuit

Toxins

Name Number Comment
None

Antagonists

Name Number Comment
Tyrosine

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG
LacI
lacI promoter
tRNA
tRNA synthetase
UAA (propargyl-L-lysine)
IPTG
lacI
lac/ara-L promoter K354000
Tyr Synthetase (modified with TAG mutation)
two resistances for selection