UT Dallas 2011

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them. Co-cultural killswitch

Toxins

Name Number Comment
Colicin BBa_K131010 depolarisation of cytoplasmic membrance and DNase- and RNase-activity

Antagonists

Name Number Comment
Glucose high levels of glucose repress the promoter PcstA

Topology

Kill Switch topology

Parts

Name Number Comment
PcstA-RBS-LuxI-terminator BBa_K569001 coding for LuxI which produces AHL in absence of glucose
AHL-inducible ColicinE2-GFP BBa_K131010 AHL-inducible Colicin production linked with GFP