Implemented kill switches: 2
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
ccdB |
|
attacks gyrase (a topoisomerase II) which results in the blockade of polymerases. |
Antagonists
Name |
Number |
Comment |
ccdA |
|
inhibits the toxic activity by forming a ccdA:ccdB complex |
Topology
Parts
Name |
Number |
Comment |
LOV2 2012 |
BBa_K820000 |
contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm). |
promoter (lacI regulated) |
BBa_R0010 |
In the absence of LacI protein and CAP protein this part promotes transcription. |
RBS |
BBa_B0032 |
Ribosom binding site |
T1 |
BBa_B0010 |
T1 from e. coli rrnB |
TE |
BBa_B0012 |
Transcription terminator for the E.coli RNA polymerase |
Kill Switch 2
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
mazF |
|
is a sequence-specific ribonuclease from B. subtilis and E. coli. |
Antagonists
Name |
Number |
Comment |
mazE |
|
counterbalance mazF |
Topology
Parts
Name |
Number |
Comment |
LOV2 2012 |
BBa_K820000 |
contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm). |
promoter (lacI regulated) |
BBa_R0010 |
In the absence of LacI protein and CAP protein this part promotes transcription. |
RBS |
BBa_B0032 |
Ribosom binding site |
T1 |
BBa_B0010 |
T1 from e. coli rrnB |
TE |
BBa_B0012 |
Transcription terminator for the E.coli RNA polymerase |
mazF |
BBa_K302033 |
Encodes a stable sequence-specific ribonuclease |