Marburg 2014

Implemented kill switches: 1

Kill Switch 1

Class

Redundant killing and mutant avoiding circuit

Toxins

Name Number Comment
T4 Holin Kills the cell
yvyD Inactivates ribosomes by dimerization
- Essential for assembly of ribosomal subunits

Antagonists

Name Number Comment
T4 Aniholin
L5

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG
LacI
TetR
All contained in lacA-flanc and amyE-flanc