LMU Munich 2012

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
MazF BBa_K823044 MazF is a bacterial toxin from E. coli and degrades mRNA.

Antagonists

Name Number Comment
mazE counterbalance mazF

Topology

Kill Switch topology

Parts

Name Number Comment
MazF BBa_K823044 MazF is a bacterial toxin from E. coli and degrades mRNA.
PspoIVB promoter BBa_K823048 PspoIVB promoter is from B.subtilis and is a sporulation promoter activated late in sporulation by sigma G in the forespore.
Alternative sigma factor ecf41 BBa_K823043 Alternative sigma factor ecf41 from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on.
PydfG promoter BBa_K823041 PydfG is a promotor from B. lincheniformes and is regulated by the alternative sigma factor ecf41Bli.
PsspK promoter BBa_K823042 PsspK promoter regulated by sigma G late in Sporulation in the forespore.