Imperial College London 2011

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them. Prevention of horizontal gene transfer through the inhibition of a plasmid-expressed toxin by genomically integrated antitoxin

Toxins

Name Number Comment
J23103 promoter - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 BBa_K515106 Cassette for constitutive expression of holin and endolysin

Antagonists

Name Number Comment
J23100 promoter - Antiholin BBa_K515104 Antiholin with insulator and RBS and double TAA terminator for genomic integration

Topology

Kill Switch topology

Parts

Name Number Comment
J23103 promoter - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 BBa_K515106 Cassette for constitutive expression of holin and endolysin
J23100 promoter - Antiholin BBa_K515004 Antiholin with insulator and RBS and double TAA terminator for genomic integration and constitutive expression