Paris Saclay 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
TetR BBa_S03518 tetracycline repressor for repression of an essential gene

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
constitive Promotor BBa_J23101 constitutive Promotor
RNA thermometer BBa_K115017 covers SD sequence - Translationinitiation at 32 deg C
cI857 BBa_K098997 cI protein
double terminator BBa_B0015 Terminator
cI Promotor BBa_R0051 Promotor is activated by cI protein
TetR BBa_S03518 Tetracyclin repressor
pTet BBa_R0040 TetR repressible promotor
essential gene e.g. adk alaS holB metG pgk tyrS