Marburg 2013

Implemented kill switches: 1

Kill Switch 1

Class

Light activated two stage recruitment

Toxins

Name Number Comment
Protease K
DNase I

Antagonists

Name Number Comment
None

Topology

Kill Switch topology

Parts

Name Number Comment
Weak & constitutive promoter Based on definitions made in Konermann et al./2013/Nature
LITE1 TALE1 + CRY2 + CIB1 + TF bZIP (light activated)
LITE2 TALE2 + CRY2 + CIB1 + TF bZIP
TALE1 binding site
TALE2 binding site