WHU China 2012

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
BglII BBa_K112106 BglII endonuclease which cleaves DNA at AGATCT sequences

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
Xylose a sugar
XylR transcriptional regulatory protein that activates gene expression after binding to xylose
BglII BBa_K112106 BglII endonuclease which cleaves DNA at AGATCT sequences
Pu promoter BBa_K625002 Promoter sequence with the recognition sequence of XylU which gets induced through the binding of XylR-xylose
YhjH catalyzes the conversion of c-di-GMP to GMP after which cells will lose their adhesion ability and become vulnerable to endonuclease