SJTU BioX Shanghai 2010

Implemented kill switches: 2

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Barnase RNase that leads to cell death but not lysis

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
constitutive promoter BBa_J23100 These promoter parts can be used to tune the expression level of constitutively expressed part
luxl produces HSL
luxR produces LuxR
plux Promoter inducible by LuxR in concert with HSL
Barnase RNase

Kill Switch 2

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Barnase RNase that leads to cell death but not lysis

Antagonists

Name Number Comment
Glucose high glucose levels repress the pBad promoter

Topology

Kill Switch topology

Parts

Name Number Comment
constitutive promoter BBa_J23100 These promoter parts can be used to tune the expression level of constitutively expressed part
pBad Arabinose inducible promoter
Barnase RNase