Washington 2010

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Toxin Tse2 BBa_K314200 A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly

Antagonists

Name Number Comment
Antitoxin Tsi2 BBa_K314201 Protein originating from Pseudomonas aeruginosa that confers immunity to the toxic protein Tse2

Topology

Kill Switch topology

Parts

Name Number Comment
TetR repressible promoter BBa_R0040 Promoter is constitutively ON and repressed by TetR
luxR repressor/activator BBa_C0062 Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL) which can induce pLux
pLux BBa_R0062 Promoter activated by LuxR in concert with HSL
Toxin Tse2 BBa_K314200 A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly
Antitoxin Tsi2 BBa_K314201 Protein originating from Pseudomonas aeruginosa that confers immunity to the toxic protein Tse2
T6SS fosmid BBa_K314300 Type VI secretion system from Pseudomonas aeruginosa in a pCC2Fos CopyControl Fosmid