UC Berkeley 2008

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
lysozyme breaks down peptidoglycan of the outer membrane
holin creates pores in the inner membrane

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
Inducible Promotor pBAD promotor - induced by arabinose
lysozyme breaks down peptidoglycan of the outer membrane
holin creates pores in the inner membrane
constitutive promotor
antiholin inhibits the activity of holin