ZJU China 2013

Implemented kill switches: 1

Kill Switch 1

Class

Induced quorum sensing leads to toxic expression Parallel circuits for holin/antiholin system

Toxins

Name Number Comment
ccdB Blocks DNA polymerases to bind to DNA through reducing gyrase
holin

Antagonists

Name Number Comment
antiholin (LrgA and LrgB)

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG Control concentration and prevent overgrowth; see paper
pLac Control concentration and prevent overgrowth; see paper
LuxR
LuxI
AHL
pLuxR
pLuxR Prevent horizontal gene transfer; see paper
CheZ Prevent horizontal gene transfer; see paper
RFP Prevent horizontal gene transfer; see paper
consecutive promoter
IrgB
IrgA
pLuxR
trzN