Vilnius Lithuania 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
crRNA

Antagonists

Name Number Comment
cI

Topology

Kill Switch topology

Parts

Name Number Comment
pLac promotor induced by IPTG
IPTG
cI cI protein
pLux/cI pLux promotor is repressed by cI protein
Cas3 Cas3 nuclease - cleaves DNA
Cd Cascade complex - guides crRNA to its target gene
crRNA CRISPR-RNA - complementary RNA to target gene (here DNA Pol III and RNA Pol)
LuxR+LuxI LuxI protein (homoserine lactone) forms a complex with LuxR which bind to the pLux promotor and activates it