Imperial College London 2009

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
DpnII BBa_K200009 This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C.
TaqI BBa_K200010 This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C.

Antagonists

Name Number Comment
clts BBa_K098997 temperature sensitive repressor of cl promoter loses binding efficiency between 35 and 42 degrees celcius
Dam BBa_K200001 prevents cleavage of DNA by leaky expression of restriction enzymes

Topology

Kill Switch topology

Parts

Name Number Comment
temperature-inducible promoter cl BBa_K098995 This is a heat sensitive cI QPI with a high promoter preceding the repressor
DpnII Composite BBa_K200009 This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C.
TaqI Composite BBa_K200010 This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C.
Dam methylase BBa_K200001 protects the DNA from cleavage
costitutive promoter BBa_J23103 constitutive promoter