USTC 2009

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
ccdB

Antagonists

Name Number Comment
tetR

Topology

Kill Switch topology

Parts

Name Number Comment
pLux-Tet promoter inhibited by tetR and activated by luxR-AHL
RBS ribosome binding site
ccdB cell cycle toxin
pCons constitutive promoter
luxI-LVA encodes for AHL
tetR-LVA represses activity of pLux-Tet promoter
pCons constitutive promoter
luxR bound with AHL - able to activate the pLux-Tet promoter