Trieste 2012

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
T4 Holin BBa_K112000 membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane
Cathelicidin LL-37 antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs

Antagonists

Name Number Comment
CymR

Topology

Kill Switch topology

Parts

Name Number Comment
pCons BBa_J23100 constitutive promotor
CymR cumate-responsive regulator CymR - can bind to T5 cumate operator
RBS
Ter
T5 cumate operator cumate responsive promotor inhibited by CymR
T4 Holin BBa_K112000 membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane
Cathelicidin LL-37 antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs