iGEM IONIS 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
HE BBa_K1616005 Holin/Endolysin

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
Iq promoter
YF1 light oxygen voltage blue light photosensor domain - in absence of blue light YF1 phosphorylates FixJ
FixJ phosphorylated FixJ imduces gene expression from the FixK2 promoter
FixK2 promoter induced by FixJ
cI CI protein - represses pR promoter
pR pR promoter - repressed by CI protein
HE BBa_K1616005 Holin/Endolysin
whole system - pDawn BBa_K1616019