Kyoto 2009

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Ura3 BBa_K210003 Marker Protein for Yeast. Expression leads to viability on medium without Uracil.

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
Timer vector #1600 for measurement BBa_K210008 This parts is LacI bindingsaites-pTEF2-EGFP::PEST-ter-CEN-ARS-pTEF2-Ura3-ter-LacI bindingsaites. Restricted by EcoR1 and Pst1 and intoroduced with BBa_K210005 you can observe fluorescence of EGFP and about 7times cell divisions later the fluorescence will disappear. We used this to evaluate Timer vector. Reference iGEM Kyoto 2009 team wiki.
LacI generator yeast-optimized BBa_K210005 This parts is consisted of pTEF2(BBa_K165037) and kozak and lacI and ADH1 terminator(BBa_K165018)