Implemented kill switches: 1
Kill Switch 1
Class
Linear line of repressors
Toxins
Name | Number | Comment |
---|---|---|
MazF | K302033 | Coding toxic protein MazF |
Antagonists
Name | Number | Comment |
---|---|---|
MazE | Not used here |
Topology
Parts
Name | Number | Comment |
---|---|---|
IPTG | ||
LacI | K1088018 | Coding lacI repressor from E. coli |
CI | C0051 | Coding cI |
Promoter 1 | J23116 | Weak constitutive promoter |
Promoter 2 | R0010 | LacI repressible promoter |
Promoter 3 | R0051 | CI repressible promoter |
Double terminator | B0015 | Double terminator |