LMU Munich 2010

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Bak inducer of apoptosis

Antagonists

Name Number Comment
Stop codon upon integration into the genome the stop codon will stop the translation in front of Bak
SV40PA upon integration into the genome the polyadenylation site will ensure the stop of translation

Topology

Kill Switch topology

Parts

Name Number Comment
CMV promoter inducible by tetracycline
attB bacterial attachment site for integration
attP phage attachment site for integration
SV40PA downstream ployadenylation site
Phic31o Integrase for integration
CMV sucrose inducible promoter