Kyoto 2010

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Holin BBa_K358004 triggers degradation of cell wall
endolysin BBa_K358004 lyses bacterial membrane

Antagonists

Name Number Comment
Antiholin BBa_K358012 Antiholin is the antitoxin against Holin. The loss of transmembrane domain 1 leads to loss of lysis function of the cassette.
Antiholin BBa_K358004 Antiholin is the antitoxin against Holin.
Lacl repressor of pLac

Topology

Kill Switch topology

Parts

Name Number Comment
Lysisbox -type2- BBa_K358021 Anti-killergene is induced constitutively and killergene is regulated by lactose promoter.