Calgary 2012

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
S7 micrococcal nuclease BBa_K902019 S7 has both endo and exonuclease activity and has a preference for -AT rich regions as opposed to -GC rich regions.
CviAII restriction endonuclease BBa_K902022 recognizes small four-base pair restriction sites.

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
TetR repressible promoter BBa_R0040 is constitutively on and repressed by TetR.
RBS BBa_B0034
S7 micrococcal nuclease BBa_K902019 S7 has both endo and exonuclease activity and has a preference for -AT rich regions as opposed to -GC rich regions.
Manganese homeostatic regulation (mntR) BBA_K902030 detects the level of manganese in the cell and acts as a transcription factor to control the expression of manganese transporter such as MntH and MntP and MntABCDE.
mntP promoter BBa_K902073 is responsible for maintaining manganese homeostasis in the cell.
mntP riboswitch BBa_K902074 The riboswitch will continue transcription in the presence of manganese and forms the hairpin structure when there is less than 10%.
CviAII restriction endonuclease BBa_K902022 recognizes small four-base pair restriction sites.