Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
S7 micrococcal nuclease |
BBa_K902019 |
S7 has both endo and exonuclease activity and has a preference for -AT rich regions as opposed to -GC rich regions. |
CviAII restriction endonuclease |
BBa_K902022 |
recognizes small four-base pair restriction sites. |
Topology
Parts
Name |
Number |
Comment |
TetR repressible promoter |
BBa_R0040 |
is constitutively on and repressed by TetR. |
RBS |
BBa_B0034 |
|
S7 micrococcal nuclease |
BBa_K902019 |
S7 has both endo and exonuclease activity and has a preference for -AT rich regions as opposed to -GC rich regions. |
Manganese homeostatic regulation (mntR) |
BBA_K902030 |
detects the level of manganese in the cell and acts as a transcription factor to control the expression of manganese transporter such as MntH and MntP and MntABCDE. |
mntP promoter |
BBa_K902073 |
is responsible for maintaining manganese homeostasis in the cell. |
mntP riboswitch |
BBa_K902074 |
The riboswitch will continue transcription in the presence of manganese and forms the hairpin structure when there is less than 10%. |
CviAII restriction endonuclease |
BBa_K902022 |
recognizes small four-base pair restriction sites. |