USTC China 2014

Implemented kill switches: 1

Kill Switch 1

Class

Direct promoter for LALF expression (Linear one-node network)

Toxins

Name Number Comment
LALF Prevents nutrition uptake and bacterial spread through biofilm formation by binding LPS

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG Needs to be added to culture to activate the switch
lactose operator
LALF K1363200 regulated by lacI
LALF K1363201 regulated by IPTG