Minnesota 2014

Implemented kill switches: 2

Kill Switch 1

Toxins

Name Number Comment
VapC Kills the cell through RNA cleavage
ParE

Antagonists

Name Number Comment
VapB
ParD

Topology

Kill Switch topology

Parts

Name Number Comment
genes for toxin/antitoxin system
genes for Pilus

Kill Switch 2

Link

Class

Linearly light induced holin/lysin production

Toxins

Name Number Comment
Holin K772101 Kills the cell by holin system
Endolysin

Antagonists

Name Number Comment
None

Topology

Kill Switch topology

Parts

Name Number Comment
VVD and blue light inducible promoter Information: http://www.nature.com/nrm/journal/v13/n4/pdf/nrm3307.pdf
gal4 (65) (inactive form of gal4)
Gal4 inducible promoter