Gaston Day 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
HE BBa_K1616005 Holin/Endolysin

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
pBAD BBa_K206000 arabinose-incuible promoter
RBS BBa_B0034 ribosime binding site
TetR BBa_C0040 Tet repressor protein
Ter BBa_B0015 Terminator
pTet BBa_R0040 repressed by Tet repressor protein
Colicin BBa_K117001 Endonuclease with random cutting site - degradation of ds/ssDNA