K.U. Leuven 2011

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
CeaB BBa_K131009 an DNase which digests host DNA and is part of the Colicin E2 operon

Antagonists

Name Number Comment
cI repressor inhibition of pLux-cI promoter
luxR BBa_I0462 inhibition of pLac-lux promoter

Topology

Kill Switch topology

Parts

Name Number Comment
cI repressor inhibition of pLux-cI promoter
luxR BBa_I0462 inhibition of pLac-lux promoter
CeaB BBa_K131009 an DNase which digests host DNA and is part of the Colicin E2 operon
Ribokey opening of ribolock
Ribolock complementary sequence on RNA due to which RNA forms a double strand which is unaccessable for the ribosome
Lactose
Arabinose
Cold shock temperature below 30 deg C