METU Ankara 2011

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Bacteriophage 21 Lysis Cassette S and R and Rz (PVJ4) BBa_K124003 consists of Holin (S) and Endolysin (R) which is the actual toxin and Rz protein

Antagonists

Name Number Comment
RNA thermometer (ROSE) BBa_K115001 RNA that binds at 37 deg C as dimer to a complementary sequence on the DNA and inhibits transkription. Linearization at 42 deg C allows transkription

Topology

Kill Switch topology

Parts

Name Number Comment
RNA thermometer (ROSE) BBa_K115001 RNA that binds at 37 deg C as dimer to a complementary sequence on the DNA and inhibits transkription. Linearization at 42 deg C allows transkription
T7 Promoter BBa_I712074 strong Promoter of Bacteriophage T7
GFP BBa_E0040 Reporterprotein to determine proteine expression levels
Bacteriophage 21 Lysis Cassette S and R and Rz (PVJ4) BBa_K124003 consists of Holin (S) and Endolysin (R) and Rz protein