ZJU-China 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
CidA lysis cell membrane

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
CI coding part BBa-C0051 Coding cI
LacI coding part BBa_K1088018 coding LacI repressor from E. coli
Promoter 1 BBa-J23116 Weak constitutive promoter for IrgA
RBS BBa_B0034 Ribosom binding site for IrgA
T1 BBa_B0010 T1 from e. coli rrnB
TE BBa_B0012 Transcription terminator for the E.coli RNA polymerase
Promoter 2 BBa-R0010 LacI repressible promoter
Promoter 3 BBa-R0051 CI repressible promoter
similar to BNU-China 2014