Berkeley UC 2007

Implemented kill switches: 2

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Barnase BBa_I716211 Barnase is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. Lethal to its cell when expressed without its inhibitor Barstar.

Antagonists

Name Number Comment
Glucose Glucose is a sugar with the molecular formula C6H12O6.

Topology

Kill Switch topology

Parts

Name Number Comment
pBAD strong BBa_K206000 Arabinose inducable promoter (http://parts.igem.org/PBAD_Promoter_Family)
Single RBS 1106A BBa_I716166 Rbosome binding site

Kill Switch 2

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
BamHI BamH I (from Bacillus amyloliquefaciens) is a type II restriction endonuclease that has the capacity for recognizing short sequences (6 b.p.) of DNA and specifically cleaving them at a target site. Lethal if expressed in cell.

Antagonists

Name Number Comment
Glucose Glucose is a sugar with the molecular formula C6H12O6.

Topology

Kill Switch topology

Parts

Name Number Comment
pBAD strong BBa_K206000 Arabinose inducable promoter (http://parts.igem.org/PBAD_Promoter_Family)
Single RBS 1106A BBa_I716166 Rbosome binding site