METU Turkey 2015

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
T4 Holin BBa_K112000 pore formation in bacterial cell membrane
T4 Endolysin BBa_K112806 degrades petidoglycan layer

Antagonists

Name Number Comment
T4 antiholin BBa_K112006 can bind T4 holin - no pore formation possible

Topology

Kill Switch topology

Parts

Name Number Comment
promoter BBa_J23106 constitutive promoter
RBS BBa_B0030 ribosome binding site
T4 antiholin BBa_K112006 can bind T4 holin - no pore formation possible
LuxR BBa_C0062
pLux BBa_I1051 LuxR induced promoter - weak constitutive expression!
T4 Holin BBa_K112000 pore formation in bacterial cell membrane
T4 Endolysin BBa_K112806 degrades petidoglycan layer