Implemented kill switches: 2
Kill Switch 1
Class
Repression of holin with multiple linearly arranged repressors
Toxins
Name |
Number |
Comment |
Holin |
|
|
Lysozyme |
K112022 |
|
Topology
Parts
Name |
Number |
Comment |
arabinose |
|
|
araC |
|
|
IPTG |
|
|
LacI |
|
|
cI repressor |
|
|
promoter |
R0051 |
|
P.A.S.E 1 |
K1223001 |
|
Kill Switch 2
Class
Disfunctional essential protein in absense of fulfillment of parallel circuit
Antagonists
Name |
Number |
Comment |
Tyrosine |
|
|
Topology
Parts
Name |
Number |
Comment |
IPTG |
|
|
LacI |
|
|
lacI promoter |
|
|
tRNA |
|
|
tRNA synthetase |
|
|
UAA (propargyl-L-lysine) |
|
|
IPTG |
|
|
lacI |
|
|
lac/ara-L promoter |
K354000 |
|
Tyr Synthetase (modified with TAG mutation) |
|
|
two resistances for selection |
|
|