Wageningen UR 2014

Implemented kill switches: 1

Kill Switch 1

Class

IO plasmid controlled by toggle switch plasmid that's externally initialized

Toxins

Name Number Comment
GFP Modular and GFP as current target gene

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
IPTG Sets the initial state
Rhamnose Replacable by other quorum sensing mechanisms
pRha Replacable by other quorum sensing mechanisms
CI Replacable by other quorum sensing mechanisms
pCL/TetR Replacable by killing mechanisms
GFP Replacable by killing mechanisms
pCI/Lac Detailed parts listing available
TetR Detailed parts listing available
pTet
LacI