TU Darmstadt 2015

Implemented kill switches: 2

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
hokD BBa_K1497008 former known as relF - interferes with a vital function in the cell membrane

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
pCons BBa_J23100 constitutive promotor
crRNA cis-repressing sequence with masked RBS
pBAD BBa_K808000 araC-regulated pBAD promotor
hokD BBa_K1497008 former known as relF - interferes with a vital function in the cell membrane
taRNA BBa_K1602049 forms a RNA-RNA complex with crRNA - RBS of crRNA is free and enables expression of GOI (hokD)

Kill Switch 2

Link

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
hokD BBa_K1497008 former known as relF - interferes with a vital function in the cell membrane

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
pCons BBa_J23100 constitutive promotor
crRNA BBa_J01080 cis-repressing sequence with masked RBS
pBAD BBa_K808000 araC-regulated pBAD promotor
hokD BBa_K1497008 former known as relF - interferes with a vital function in the cell membrane
taRNA BBa_J01086 forms a RNA-RNA complex with crRNA - RBS of crRNA is free and enables expression of GOI (hokD)