ULB Brussels 2010

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
parE Toxin of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE

Antagonists

Name Number Comment
ParD ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE
3-oxo-hexanoyl-homoserine lactone BBa_C0061 HSL inhibits the promotor of the toxin plasmid
LuxR BBa_C0062 LuxR inhibits the promotor of the toxin plasmid

Topology

Kill Switch topology

Parts

Name Number Comment
lux pR BBa_R0062 Promoter activated by LuxR in concert with HSL
luxl BBa_C0061 Synthesizes 3OC6HSL which binds to LuxR
luxR BBa_C0062 In complex with HSL the LuxR binds to the Lux promoter
double terminator BBa_B0015 Double terminator consisting of BBa_B0010 and BBa_B0012
parD ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE
HSL-mediated luxR repressor BBa_R0061 Promoter that is inhibited by HSL and LuxR
aiiA BBa_C0060 encodes an enzyme that catalyzes the degradation of N-acyl-homoserine lactones/HSL
parE Toxin of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE