METU Gene 2009

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them.

Toxins

Name Number Comment
Granulysin (a T Cell Product) BBa_K258002 Kills Bacteria by Altering Membrane Permeability. A protein located in the acidic granules of human NK cells and cytotoxic T cells and it has antimicrobial activity against a broad spectrum of microbial pathogens. Granulysin increased the permeability of bacterial membranes as judged by its ability to allow access of cytosolic beta-galactosidase to its impermeant substrate. It functions to create holes in the target cell membrane and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.

Antagonists

Name Number Comment

Topology

Kill Switch topology

Parts

Name Number Comment
p_qrr4 from Vibrio harveyi BBa_K131017 qrr4 promoter from Vibrio harveyi
cI repressor from E. coli phage lambda (+LVA) BBa_C0051 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
promoter (lambda cI regulated) BBa_R0051 The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
autoinducer synthetase for AHL BBa_C0061 Synthesizes 3OC6HSL which binds to LuxR.
luxR repressor/activator BBa_C0062 In complex with HSL LuxR binds to the Lux promoter and activates transcription from Pr BBa_R0062 and repressing transcription from Pl BBa_R0063.