VIT Vellore 2011

Implemented kill switches: 1

Kill Switch 1

Class

# Format: # Write how you would classify them. inducible toxin production

Toxins

Name Number Comment
Holin BBa_K124014 forms pores in the cell membrane

Antagonists

Name Number Comment
Glucose PcstA is repressed by high levels of glucose

Topology

Kill Switch topology

Parts

Name Number Comment
PcstA BBa_K118011 glucose-repressible promoter which is induced by cAMP
RBS.1 (strong) BBa_B0030 Ribosome binding site
pS105 BBa_K124014 Encoding for bacteriophage Holin
T7TE-LuxIA BBa_B0014 double terminator