Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
Ura3 |
BBa_K210003 |
Marker Protein for Yeast. Expression leads to viability on medium without Uracil. |
Topology
Parts
Name |
Number |
Comment |
Timer vector #1600 for measurement |
BBa_K210008 |
This parts is LacI bindingsaites-pTEF2-EGFP::PEST-ter-CEN-ARS-pTEF2-Ura3-ter-LacI bindingsaites. Restricted by EcoR1 and Pst1 and intoroduced with BBa_K210005 you can observe fluorescence of EGFP and about 7times cell divisions later the fluorescence will disappear. We used this to evaluate Timer vector. Reference iGEM Kyoto 2009 team wiki. |
LacI generator yeast-optimized |
BBa_K210005 |
This parts is consisted of pTEF2(BBa_K165037) and kozak and lacI and ADH1 terminator(BBa_K165018) |