Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
Name |
Number |
Comment |
DpnII |
BBa_K200009 |
This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C. |
TaqI |
BBa_K200010 |
This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C. |
Antagonists
Name |
Number |
Comment |
clts |
BBa_K098997 |
temperature sensitive repressor of cl promoter loses binding efficiency between 35 and 42 degrees celcius |
Dam |
BBa_K200001 |
prevents cleavage of DNA by leaky expression of restriction enzymes |
Topology
Parts
Name |
Number |
Comment |
temperature-inducible promoter cl |
BBa_K098995 |
This is a heat sensitive cI QPI with a high promoter preceding the repressor |
DpnII Composite |
BBa_K200009 |
This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C. |
TaqI Composite |
BBa_K200010 |
This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C. |
Dam methylase |
BBa_K200001 |
protects the DNA from cleavage |
costitutive promoter |
BBa_J23103 |
constitutive promoter |