LOCUS       Part2_1                 5419 bp ds-DNA     circular     09-JUN-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(5140..5419,1..1790)
                     /label=pSB1C3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1745..2493
                     /label=Part2-1-1
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1769..1790
                     /label=RFC[10] Prefix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1651..1670
                     /label=VF2 (BBa_G00100)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2464..3362
                     /label=Part2-1-2
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3333..4231
                     /label=Part2-1-3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4202..5189
                     /label=Part2-1-4
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    5140..5160
                     /label=RFC[10] Suffix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5294..5313)
                     /label=VR (BBa_G00101)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2809..3912
                     /label=(Phosphomevalonate kinase; Enterococcus faecalis)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1873..2775
                     /label=mvk (Mevalonate kinase; Methanosarcina mazei)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3946..5133
                     /label=mvd1 (Diphosphomevalonate decarboxylase;
                     Saccharomyces cerevisiae)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1852..1866
                     /label=BBa_B0030
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             2788..2802
                     /label=BBa_B0030(1)
                     /ApEinfo_label="BBa_B0030"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             3925..3939
                     /label=BBa_B0030(2)
                     /ApEinfo_label="BBa_B0030"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1791..1845
                     /label=BBa_R0011
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
       61 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      421 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      721 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
      781 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      841 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      901 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      961 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1021 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1081 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     1141 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1201 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1261 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1321 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1381 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1441 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1501 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1561 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1621 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     1681 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1741 ccttagcttt cgctaaggat gatttctggA attcgcggcc gcttctagag aattgtgagc
     1801 ggataacaat tgacattgtg agcggataac aagatactga gcacatacta gattaaagag
     1861 gagaaatact agATGGTGAG TTGTTCAGCT CCCGGCAAAA TTTATCTGTT CGGCGAACAC
     1921 GCCGTGGTCT ATGGAGAAAC TGCGATTGCT TGCGCAGTGG AATTACGTAC TCGCGTACGT
     1981 GCAGAACTGA ACGACAGCAT CACTATCCAA AGCCAGATTG GACGCACGGG TCTGGACTTC
     2041 GAAAAGCACC CTTACGTGAG CGCAGTCATT GAGAAAATGC GGAAGAGTAT CCCGATTAAT
     2101 GGCGTATTCC TCACTGTCGA TAGTGATATT CCGGTTGGAA GTGGGCTGGG TTCATCCGCC
     2161 GCAGTCACGA TCGCCTCTAT CGGCGCGTTA AATGAGCTTT TTGGTTTCGG CCTGAGCCTG
     2221 CAAGAAATTG CTAAATTAGG GCATGAGATT GAAATTAAAG TGCAGGGCGC TGCGAGCCCT
     2281 ACCGATACGT ATGTTTCAAC CTTTGGTGGC GTTGTGACGA TCCCGGAACG CCGTAAACTG
     2341 AAGACCCCTG ATTGCGGCAT TGTCATCGGT GACACAGGCG TGTTCTCGTC AACTAAAGAA
     2401 CTGGTGGCAA ACGTGCGTCA GTTACGTGAA TCGTATCCAG ATCTGATTGA ACCTCTGATG
     2461 ACGAGCATTG GGAAAATTAG TCGTATTGGC GAGCAACTTG TGCTGAGTGG TGATTACGCA
     2521 AGTATTGGCC GTTTGATGAA TGTGAATCAG GGTCTGCTGG ATGCGCTGGG CGTGAACATC
     2581 CTCGAACTGA GCCAACTCAT TTATAGTGCC CGGGCCGCGG GCGCATTCGG CGCCAAGATT
     2641 ACAGGCGCCG GTGGTGGGGG CTGCATGGTG GCGCTTACCG CCCCCGAGAA GTGCAATCAG
     2701 GTTGCTGAAG CGGTTGCAGG TGCGGGTGGT AAAGTGACGA TTACGAAACC GACGGAGCAG
     2761 GGGCTGAAGG TAGACTAATA Atactagatt aaagaggaga aatactagAT GATTGAGGTT
     2821 ACCACCCCGG GGAAGCTGTT CATCGCTGGC GAATACGCGG TCGTTGAACC GGGTCACCCG
     2881 GCTATCATTG TGGCCGTGGA TCAATTTGTG ACCGTTACAG TCGAGGAGAC TACTGATGAA
     2941 GGCTCGATCC AGAGCGCACA ATACTCATCC CTTCCGATTC GCTGGACACG TCGCAATGGT
     3001 GAACTGGTTC TTGATATCCG TGAAAATCCT TTTCATTATG TGCTCGCAGC CATCCATCTT
     3061 ACCGAAAAAT ACGCACAGGA ACAAAACAAG GAACTGTCAT TCTATCATTT GAAAGTCACC
     3121 AGCGAATTGG ATAGTAGCAA TGGCCGTAAG TATGGACTCG GCTCTAGTGG TGCAGTAACC
     3181 GTAGGAACGG TAAAAGCATT AAACATTTTT TACGATCTGG GTTTGGAGAA CGAAGAAATT
     3241 TTTAAACTGT CAGCCTTAGC ACACCTGGCG GTGCAAGGTA ACGGATCATG TGGAGATATC
     3301 GCCGCATCCT GTTATGGTGG CTGGATTGCC TTTTCAACCT TTGACCATGA TTGGGTCAAC
     3361 CAAAAAGTGG CAACAGAGAC GTTAACGGAT CTTCTGGCGA TGGACTGGCC GGAACTTATG
     3421 ATTTTCCCGC TGAAGGTTCC TAAGCAATTG CGTTTACTGA TCGGCTGGAC GGGTTCGCCA
     3481 GCTAGTACAA GCGATCTGGT GGATCGTGTA CATCAGTCCA AGGAGGAAAA ACAAGCGGCC
     3541 TACGAACAGT TCCTTATGAA ATCACGCTTG TGTGTGGAGA CGATGATCAA TGGGTTTAAC
     3601 ACGGGGAAAA TCAGTGTGAT TCAAAAGCAG ATTACGAAGA ACCGCCAACT GTTAGCTGAG
     3661 TTAAGTTCCC TCACCGGGGT CGTCATCGAA ACAGAAGCAC TCAAAAATCT CTGCGACCTG
     3721 GCCGAAAGCT ACACTGGGGC CGCAAAATCC AGTGGCGCGG GCGGTGGGGA CTGCGGAATT
     3781 GTTATCTTTC GTCAGAAATC TGGTATTTTA CCGCTGATGA CCGCATGGGA AAAAGACGGG
     3841 ATTACCCCCC TGCCCCTGCA TGTGTACACC TATGGACAGA AGGAGTGTAA AGAGAAACAC
     3901 GAATCAAAAC GTTAATAAta ctagattaaa gaggagaaat actagATGAC GGTGTATACT
     3961 GCATCGGTAA CCGCCCCGGT CAACATCGCG ACACTGAAAT ACTGGGGCAA ACGTGATACC
     4021 AAACTGAACT TACCGACCAA CTCTTCGATC TCGGTTACCC TGTCTCAAGA CGATTTGCGT
     4081 ACGTTAACCT CGGCCGCGAC TGCTCCGGAA TTTGAACGCG ACACGCTCTG GCTGAACGGT
     4141 GAACCGCACA GCATCGATAA TGAACGCACC CAGAATTGCT TGCGTGATCT GCGCCAGCTG
     4201 CGCAAAGAGA TGGAATCGAA AGATGCCAGC CTGCCTACTC TGTCTCAATG GAAGCTGCAT
     4261 ATTGTTTCCG AAAACAATTT TCCGACGGCG GCAGGTCTCG CCAGCTCAGC AGCAGGTTTC
     4321 GCGGCATTGG TCAGTGCTAT CGCGAAACTG TACCAACTCC CGCAATCAAC GAGTGAAATT
     4381 AGCCGCATCG CCCGTAAAGG GAGTGGGTCA GCATGTCGTT CTTTATTCGG AGGTTATGTG
     4441 GCCTGGGAAA TGGGCAAAGC AGAAGATGGC CATGACAGTA TGGCGGTGCA AATCGCTGAT
     4501 TCCAGCGACT GGCCGCAAAT GAAGGCTTGT GTGCTGGTTG TGTCCGATAT TAAGAAAGAT
     4561 GTAAGCAGTA CCCAGGGTAT GCAGTTGACT GTCGCGACCA GCGAACTGTT TAAAGAACGT
     4621 ATTGAACATG TGGTGCCGAA ACGTTTTGAA GTAATGCGCA AAGCAATTGT TGAGAAAGAC
     4681 TTTGCGACCT TTGCCAAAGA AACGATGATG GATTCTAACT CTTTTCACGC AACCTGCCTT
     4741 GACAGCTTTC CACCGATCTT CTACATGAAC GATACGTCGA AACGCATTAT TAGTTGGTGC
     4801 CACACGATTA ACCAGTTCTA TGGTGAGACA ATTGTGGCCT ATACGTTCGA CGCGGGACCC
     4861 AACGCAGTTT TATATTACCT GGCAGAAAAT GAAAGTAAGC TGTTTGCCTT TATCTACAAG
     4921 TTGTTCGGCT CAGTGCCGGG CTGGGACAAG AAATTTACCA CTGAACAGTT GGAAGCCTTC
     4981 AATCACCAGT TTGAATCTTC GAACTTCACG GCGCGCGAGC TGGATCTCGA ACTGCAAAAA
     5041 GATGTCGCAC GTGTTATTCT GACCCAGGTT GGCTCTGGGC CGCAGGAAAC CAATGAATCA
     5101 CTTATTGATG CTAAAACGGG CCTGCCGAAA GAGTAATAAt actagtagcg gccgctgcag
     5161 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     5221 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5401 gttgctggcg tttttccac    
//
